TY  - JOUR
AU  - Muschallik, Lukas
AU  - Molinnus, Denise
AU  - Jablonski, Melanie
AU  - Kipp, Carina Ronja
AU  - Bongaerts, Johannes
AU  - Pohl, Martina
AU  - Wagner, Torsten
AU  - Schöning, Michael J.
AU  - Selmer, Thorsten
AU  - Siegert, Petra
TI  - Synthesis of α-hydroxy ketones and vicinal ( R , R )-diols by Bacillus clausii DSM 8716 T butanediol dehydrogenase
JO  - RSC Advances
VL  - 10
IS  - 21
SN  - 2046-2069
CY  - London
PB  - RSC Publishing
M1  - FZJ-2020-01831
SP  - 12206 - 12216
PY  - 2020
AB  - α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716T (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn2+ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000526839700010
DO  - DOI:10.1039/D0RA02066D
UR  - https://juser.fz-juelich.de/record/875139
ER  -