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@ARTICLE{Jansen:875247,
      author       = {Jansen, Roman P. and Müller, Moritz-Fabian and Schröter,
                      Sonja and Kappelmann, Jannick and Klein, Bianca and Oldiges,
                      Marco and Noack, Stephan},
      title        = {{P}arallelized disruption of prokaryotic and eukaryotic
                      cells via miniaturized and automated bead mill},
      journal      = {Engineering in life sciences},
      volume       = {20},
      number       = {8},
      issn         = {1618-2863},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2020-01894},
      pages        = {350-356},
      year         = {2020},
      abstract     = {The application of integrated microbioreactor systems is
                      rapidly becoming of more interest to accelerate strain
                      characterization and bioprocess development. However,
                      available high‐throughput screening capabilities are often
                      limited to target extracellular compounds only.
                      Consequently, there is a great demand for automated
                      technologies allowing for miniaturized and parallel cell
                      disruption providing access to intracellular measurements.
                      In this study, a fully automated bead mill workflow was
                      developed and validated for four different industrial
                      platform organisms: Escherichia coli , Corynebacterium
                      glutamicum , Saccharomyces cerevisiae , and Aspergillus
                      niger . The workflow enables up to 48 parallel cell
                      disruptions in microtiter plates and is applicable at‐line
                      to running lab‐scale cultivations. The resulting cell
                      extracts form the basis for quantitative omics studies where
                      no rapid metabolic quenching is required (e.g., genomics and
                      proteomics).},
      cin          = {IBG-1},
      ddc          = {660},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32774207},
      UT           = {WOS:000530629000001},
      doi          = {10.1002/elsc.202000002},
      url          = {https://juser.fz-juelich.de/record/875247},
}