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@ARTICLE{Grieschat:875317,
      author       = {Grieschat, Matthias and Guzman, Raul E and Langschwager,
                      Katharina and Fahlke, Christoph and Alekov, Alexi K},
      title        = {{M}etabolic energy sensing by mammalian {CLC} anion/proton
                      exchangers},
      journal      = {EMBO reports},
      volume       = {21},
      number       = {6},
      issn         = {1469-3178},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {FZJ-2020-01948},
      pages        = {e47872},
      year         = {2020},
      abstract     = {CLC anion/proton exchangers control the pH and [Cl−] of
                      the endolysosomal system that is essential for cellular
                      nutrient uptake. Here, we use heterologous expression and
                      whole‐cell electrophysiology to investigate the regulation
                      of the CLC isoforms ClC‐3, ClC‐4, and ClC‐5 by the
                      adenylic system components ATP , ADP , and AMP . Our results
                      show that cytosolic ATP and ADP but not AMP and Mg2+‐free
                      ADP enhance CLC ion transport. Biophysical analysis reveals
                      that adenine nucleotides alter the ratio between CLC ion
                      transport and CLC gating charge and shift the CLC
                      voltage‐dependent activation. The latter effect is
                      suppressed by blocking the intracellular entrance of the
                      proton transport pathway. We suggest, therefore, that
                      adenine nucleotides regulate the internal proton delivery
                      into the CLC transporter machinery and alter the probability
                      of CLC transporters to undergo silent non‐transporting
                      cycles. Our findings suggest that the CBS domains in
                      mammalian CLC transporters serve as energy sensors that
                      regulate vesicular Cl−/H+ exchange by detecting changes in
                      the cytosolic ATP /ADP /AMP equilibrium. Such sensing
                      mechanism links the endolysosomal activity to the cellular
                      metabolic state.},
      cin          = {IBI-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-1-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32390228},
      UT           = {WOS:000531272100001},
      doi          = {10.15252/embr.201947872},
      url          = {https://juser.fz-juelich.de/record/875317},
}