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@MASTERSTHESIS{Glass:875396,
      author       = {Glass, Manuel},
      title        = {{M}olecular layer deposition and protein interface
                      patterning for guided cell growth},
      volume       = {215},
      school       = {Universität Köln},
      type         = {Masterarbeit},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2020-02005},
      isbn         = {978-3-95806-463-8},
      series       = {Schriften des Forschungszentrums Jülich. Reihe
                      Schlüsseltechnologien / Key Technologies},
      pages        = {IV, 81 S.},
      year         = {2020},
      note         = {Universität Köln, Masterarbeit, 2020},
      abstract     = {This thesis describes the design, assembly and structural
                      and functional characterization, of bio-(medical) applicable
                      interfacial layers with molecular controlled architectures
                      on solid substrates. The interaction between the living
                      world of cells, tissue, or whole organisms and the (organic
                      orinorganic) materials world of technical devices such as
                      implants, sensors, or medical parts requires a proper
                      construction and detailed structural (and functional)
                      control of this organism-machine interface. Therefore, a
                      possible way how to get from an optimal molecular layer
                      deposition (MLD) to guided cell growth is developed in this
                      work. By integrating a heater to an already existing MLD
                      setup and an optimization of the deposition temperature we
                      could improve the gas phase deposition process of GLYMO
                      (3-Glycidyloxypropyl)-trimethoxysilane) yielding a faster
                      formation of self-assembled monolayers(SAMs) and a better
                      quality of GLYMO SAMs. This was confirmed by ex-situ
                      analysis, e.g. fluorescence microscopy, referenced
                      ellipsometry, and surface potential measurements. With the
                      gas phase MLD, lithography, and lift-off processes
                      functionalization of SiO$_{2}$ surfaces with GLYMO SAMs and
                      patterned ploy-L-lysine proteins (PLL) could be achieved.
                      This enables to generate various micropatterns that support
                      cell adhesion, neurite outgrowth, and the formation of a
                      geometrically defined networks of neurons. Finally, guided
                      growth was demonstrated via rat cortical neuron cultures on
                      the GLYMO-PLL patterned surfaces. On first sight, the neuron
                      growth was clearly guided, i.e. neurons grow on PLL but not
                      on GLYMO. However, we also noticed that on certain areas
                      which should be coated with PLL, no cells were present. It
                      seemed that in these areas during the lithography PMMA is
                      cracked due to the e-beam exposure and partially binds to
                      the GLYMO. This cracked PMMA hinders the PLL to bind to
                      GLYMO and therefore only in places, where PLL dries out
                      during the coating, PLL is present in the GLYMO-PLL pattern.
                      These effects are observed via fluorescence imaging for the
                      PLL coating and for the cell growth. In conclusion, the
                      modified deposition process at elevated temperatures in
                      combination with the developed interface pattering process
                      via a combination of a molecular layer of GLYMO and the
                      protein PLL might be suitable for guidance of neuronal
                      growth, despite the problem of a PMMA blocking layer which
                      seem to be generated during the lithography. This
                      shortcoming could be overcome by an additional step in which
                      the blocking layer is remove.},
      cin          = {ICS-8},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)19},
      urn          = {urn:nbn:de:0001-2020060521},
      url          = {https://juser.fz-juelich.de/record/875396},
}