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@ARTICLE{Stengelin:875439,
author = {Stengelin, Elena and Kuzmina, Alena and Beltramo, Guillermo
L. and Koziol, Martha F. and Besch, Laura and Schröder,
Romina and Unger, Ronald E. and Tremel, Wolfgang and
Seiffert, Sebastian},
title = {{B}one {S}caffolds {B}ased on {D}egradable
{V}aterite/{PEG}‐{C}omposite {M}icrogels},
journal = {Advanced healthcare materials},
volume = {9},
number = {11},
issn = {2192-2659},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {FZJ-2020-02034},
pages = {1901820},
year = {2020},
abstract = {Vaterite, a metastable modification of calcium carbonate,
embedded in a flexible microgel packaging with adjustable
mechanical properties, functionality, and biocompatibility,
provides a powerful scaffolding for bone tissue
regeneration, as it is easily convertible to bone‐like
hydroxyapatite (HA). In this study, the synthesis and
physical analysis of a packaging material to encapsulate
vaterite particles and osteoblast cells into monodisperse,
sub‐millimeter‐sized microgels, is described whereby a
systematic approach is used to tailor the microgel
properties. The size and shape of the microgels is
controlled via droplet‐based microfluidics. Key
requirements for the polymer system, such as absence of
cytotoxicity as well as biocompatibility and
biodegradability, are accomplished with functionalized
poly(ethylene glycol) (PEG), which reacts in a
cytocompatible thiol–ene Michael addition. On a mesoscopic
level, the microgel stiffness and gelation times are
adjusted to obtain high cellular viabilities. The
co‐encapsulation of living cells provides i) an in vitro
platform for the study of cellular metabolic processes which
can be applied to bone formation and ii) an in vitro
foundation for novel tissue‐regenerative therapies.
Finally, the degradability of the microgels at physiological
conditions caused by hydrolysis‐sensitive ester groups in
the polymer network is examined.},
cin = {IBI-2},
ddc = {610},
cid = {I:(DE-Juel1)IBI-2-20200312},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32378355},
UT = {WOS:000530631000001},
doi = {10.1002/adhm.201901820},
url = {https://juser.fz-juelich.de/record/875439},
}