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@ARTICLE{Pietschmann:877230,
      author       = {Pietschmann, Jan and Spiegel, Holger and Krause,
                      Hans-Joachim and Schillberg, Stefan and Schröper, Florian},
      title        = {{S}ensitive {A}flatoxin {B}1 {D}etection {U}sing
                      {N}anoparticle-{B}ased {C}ompetitive {M}agnetic
                      {I}mmunodetection},
      journal      = {Toxins},
      volume       = {12},
      number       = {5},
      issn         = {2072-6651},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2020-02057},
      pages        = {337 -},
      year         = {2020},
      abstract     = {Food and crop contaminations with mycotoxins are a severe
                      health risk for consumers and cause high economic losses
                      worldwide. Currently, different chromatographic- and
                      immunobased methods are used to detect mycotoxins within
                      different sample matrices. There is a need for novel, highly
                      sensitive detection technologies that avoid time-consuming
                      procedures and expensive laboratory equipment but still
                      provide sufficient sensitivity to achieve the mandated
                      detection limit for mycotoxin content. Here we describe a
                      novel, highly sensitive, and portable aflatoxin B1 detection
                      approach using competitive magnetic immunodetection (cMID).
                      As a reference method, a competitive ELISA optimized by
                      checkerboard titration was established. For the novel cMID
                      procedure, immunofiltration columns, coated with aflatoxin
                      B1-BSA conjugate were used for competitive enrichment of
                      biotinylated aflatoxin B1-specific antibodies. Subsequently,
                      magnetic particles functionalized with streptavidin can be
                      applied to magnetically label retained antibodies. By means
                      of frequency mixing technology, particles were detected and
                      quantified corresponding to the aflatoxin content in the
                      sample. After the optimization of assay conditions, we
                      successfully demonstrated the new competitive magnetic
                      detection approach with a comparable detection limit of 1.1
                      ng aflatoxin B1 per ml sample to the cELISA reference
                      method. Our results indicate that thecMID is a promising
                      method reducing the risks of processing contaminated
                      commodities.},
      cin          = {IBI-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
      pid          = {G:(DE-HGF)POF3-523},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32443933},
      UT           = {WOS:000541799400017},
      doi          = {10.3390/toxins12050337},
      url          = {https://juser.fz-juelich.de/record/877230},
}