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@ARTICLE{Yuan:877330,
author = {Yuan, Xiaobo and Wolf, Nikolaus and Hondrich, Timm and
Shokoohimehr, Pegah and Milos, Frano and Glass, Manuel and
Mayer, Dirk and Maybeck, Vanessa and Prömpers, Michael and
Offenhäusser, Andreas and Wördenweber, Roger},
title = {{E}ngineering {B}iocompatible {I}nterfaces via
{C}ombinations of {O}xide {F}ilms and {O}rganic
{S}elf-{A}ssembled {M}onolayers},
journal = {ACS applied materials $\&$ interfaces},
volume = {12},
number = {14},
issn = {1944-8252},
address = {Washington, DC},
publisher = {Soc.},
reportid = {FZJ-2020-02146},
pages = {17121 - 17129},
year = {2020},
abstract = {In this paper, we demonstrate that cell adhesion and neuron
maturation can be guided by patterned oxide surfaces
functionalized with organic molecular layers. It is shown
that the difference in the surface potential of various
oxides (SiO2, Ta2O5, TiO2, and Al2O3) can be increased by
functionalization with a silane,
(3-aminopropyl)-triethoxysilane (APTES), which is deposited
from the gas phase on the oxide. Furthermore, it seems that
only physisorbed layers (no chemical binding) can be
achieved for some oxides (Ta2O5 and TiO2), whereas
self-assembled monolayers (SAM) form on other oxides (SiO2
and Al2O3). This does not only alter the surface potential
but also affects the neuronal cell growth. The already high
cell density on SiO2 is increased further by the chemically
bound APTES SAM, whereas the already low cell density on
Ta2O5 is even further reduced by the physisorbed APTES
layer. As a result, the cell density is ∼8 times greater
on SiO2 compared to Ta2O5, both coated with APTES.
Furthermore, neurons form the typical networks on SiO2,
whereas they tend to cluster to form neurospheres on Ta2O5.
Using lithographically patterned Ta2O5 layers on SiO2
substrates functionalized with APTES, the guided growth can
be transferred to complex patterns. Cell cultures and
molecular layers can easily be removed, and the cell
experiment can be repeated after functionalization of the
patterned oxide surface with APTES. Thus, the combination of
APTES-functionalized patterned oxides might offer a
promising way of achieving guided neuronal growth on robust
and reusable substrates.},
cin = {IBI-3},
ddc = {600},
cid = {I:(DE-Juel1)IBI-3-20200312},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32186363},
UT = {WOS:000526583500121},
doi = {10.1021/acsami.0c02141},
url = {https://juser.fz-juelich.de/record/877330},
}