000877352 001__ 877352
000877352 005__ 20210130005020.0
000877352 0247_ $$2doi$$a10.1093/jxb/eraa263
000877352 0247_ $$2ISSN$$a0022-0957
000877352 0247_ $$2ISSN$$a1460-2431
000877352 0247_ $$2Handle$$a2128/25768
000877352 0247_ $$2altmetric$$aaltmetric:82930219
000877352 0247_ $$2pmid$$apmid:32459850
000877352 0247_ $$2WOS$$aWOS:000577073500004
000877352 037__ $$aFZJ-2020-02159
000877352 082__ $$a580
000877352 1001_ $$0P:(DE-Juel1)180850$$aDumschott, Kathryn$$b0$$eCorresponding author
000877352 245__ $$aOxford Nanopore Sequencing: New opportunities for plant genomics?
000877352 260__ $$aOxford$$bOxford Univ. Press$$c2020
000877352 3367_ $$2DRIVER$$aarticle
000877352 3367_ $$2DataCite$$aOutput Types/Journal article
000877352 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1601042946_15197
000877352 3367_ $$2BibTeX$$aARTICLE
000877352 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000877352 3367_ $$00$$2EndNote$$aJournal Article
000877352 520__ $$aDNA sequencing was dominated by Sanger’s chain-termination method until the mid-2000s, when it was progressively supplanted by new sequencing technologies that can generate much larger quantities of data in a shorter time. At the forefront of these developments, long-read sequencing technologies (third-generation sequencing) can produce reads that are several kilobases in length. This greatly improves the accuracy of genome assemblies by spanning the highly-repetitive segments that cause difficulty for second-generation short-read technologies. Third-generation sequencing is especially appealing for plant genomes, which can be extremely large with long stretches of highly-repetitive DNA. Until recently, the low basecalling accuracy of third-generation technologies meant that accurate genome assembly required expensive, highcoverage sequencing followed by computational analysis to correct for errors. However, today’s long-read technologies are more accurate and less expensive, making them the method of choice for the assembly of complex genomes. Oxford Nanopore Technologies (ONT), a thirdgeneration platform for the sequencing of native DNA strands, is particularly suitable for the generation of high-quality assemblies of highly-repetitive plant genomes. Here we discuss the benefits of ONT, especially for the plant science community, and describe the issues that remain to be addressed when using ONT for plant genome sequencing.
000877352 536__ $$0G:(DE-HGF)POF3-582$$a582 - Plant Science (POF3-582)$$cPOF3-582$$fPOF III$$x0
000877352 536__ $$0G:(DE-HGF)POF3-583$$a583 - Innovative Synergisms (POF3-583)$$cPOF3-583$$fPOF III$$x1
000877352 588__ $$aDataset connected to CrossRef
000877352 7001_ $$0P:(DE-Juel1)180784$$aSchmidt, Maximilian$$b1$$ufzj
000877352 7001_ $$0P:(DE-HGF)0$$aChawla, Harmeet Singh$$b2
000877352 7001_ $$0P:(DE-HGF)0$$aSnowdon, Rod$$b3
000877352 7001_ $$0P:(DE-Juel1)145719$$aUsadel, Björn$$b4
000877352 773__ $$0PERI:(DE-600)1466717-4$$a10.1093/jxb/eraa263$$gp. eraa263$$n18$$p5313–5322$$tThe journal of experimental botany$$v71$$x1460-2431$$y2020
000877352 8564_ $$uhttps://juser.fz-juelich.de/record/877352/files/eraa263.pdf$$yOpenAccess
000877352 8564_ $$uhttps://juser.fz-juelich.de/record/877352/files/eraa263.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000877352 909CO $$ooai:juser.fz-juelich.de:877352$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000877352 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)180850$$aForschungszentrum Jülich$$b0$$kFZJ
000877352 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)180784$$aForschungszentrum Jülich$$b1$$kFZJ
000877352 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)145719$$aForschungszentrum Jülich$$b4$$kFZJ
000877352 9131_ $$0G:(DE-HGF)POF3-582$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vPlant Science$$x0
000877352 9131_ $$0G:(DE-HGF)POF3-583$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vInnovative Synergisms$$x1
000877352 9141_ $$y2020
000877352 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bJ EXP BOT : 2018$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000877352 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000877352 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bJ EXP BOT : 2018$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0430$$2StatID$$aNational-Konsortium$$d2020-01-05$$wger
000877352 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2020-01-05
000877352 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2020-01-05$$wger
000877352 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2020-01-05
000877352 920__ $$lyes
000877352 9201_ $$0I:(DE-Juel1)IBG-4-20200403$$kIBG-4$$lBioinformatik$$x0
000877352 980__ $$ajournal
000877352 980__ $$aVDB
000877352 980__ $$aUNRESTRICTED
000877352 980__ $$aI:(DE-Juel1)IBG-4-20200403
000877352 9801_ $$aFullTexts