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@ARTICLE{Dumschott:877352,
      author       = {Dumschott, Kathryn and Schmidt, Maximilian and Chawla,
                      Harmeet Singh and Snowdon, Rod and Usadel, Björn},
      title        = {{O}xford {N}anopore {S}equencing: {N}ew opportunities for
                      plant genomics?},
      journal      = {The journal of experimental botany},
      volume       = {71},
      number       = {18},
      issn         = {1460-2431},
      address      = {Oxford},
      publisher    = {Oxford Univ. Press},
      reportid     = {FZJ-2020-02159},
      pages        = {5313–5322},
      year         = {2020},
      abstract     = {DNA sequencing was dominated by Sanger’s
                      chain-termination method until the mid-2000s, when it was
                      progressively supplanted by new sequencing technologies that
                      can generate much larger quantities of data in a shorter
                      time. At the forefront of these developments, long-read
                      sequencing technologies (third-generation sequencing) can
                      produce reads that are several kilobases in length. This
                      greatly improves the accuracy of genome assemblies by
                      spanning the highly-repetitive segments that cause
                      difficulty for second-generation short-read technologies.
                      Third-generation sequencing is especially appealing for
                      plant genomes, which can be extremely large with long
                      stretches of highly-repetitive DNA. Until recently, the low
                      basecalling accuracy of third-generation technologies meant
                      that accurate genome assembly required expensive,
                      highcoverage sequencing followed by computational analysis
                      to correct for errors. However, today’s long-read
                      technologies are more accurate and less expensive, making
                      them the method of choice for the assembly of complex
                      genomes. Oxford Nanopore Technologies (ONT), a
                      thirdgeneration platform for the sequencing of native DNA
                      strands, is particularly suitable for the generation of
                      high-quality assemblies of highly-repetitive plant genomes.
                      Here we discuss the benefits of ONT, especially for the
                      plant science community, and describe the issues that remain
                      to be addressed when using ONT for plant genome sequencing.},
      cin          = {IBG-4},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-4-20200403},
      pnm          = {582 - Plant Science (POF3-582) / 583 - Innovative
                      Synergisms (POF3-583)},
      pid          = {G:(DE-HGF)POF3-582 / G:(DE-HGF)POF3-583},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32459850},
      UT           = {WOS:000577073500004},
      doi          = {10.1093/jxb/eraa263},
      url          = {https://juser.fz-juelich.de/record/877352},
}