000877541 001__ 877541
000877541 005__ 20220930130241.0
000877541 0247_ $$2doi$$a10.3389/fbioe.2020.00551
000877541 0247_ $$2Handle$$a2128/25078
000877541 0247_ $$2altmetric$$aaltmetric:83908848
000877541 0247_ $$2pmid$$apmid:32596224
000877541 0247_ $$2WOS$$aWOS:000543816700001
000877541 037__ $$aFZJ-2020-02277
000877541 082__ $$a570
000877541 1001_ $$0P:(DE-HGF)0$$aKöbbing, Sebastian$$b0
000877541 245__ $$aCharacterization of Context-Dependent Effects on Synthetic Promoters
000877541 260__ $$aLausanne$$bFrontiers Media$$c2020
000877541 3367_ $$2DRIVER$$aarticle
000877541 3367_ $$2DataCite$$aOutput Types/Journal article
000877541 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1592394919_13935
000877541 3367_ $$2BibTeX$$aARTICLE
000877541 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000877541 3367_ $$00$$2EndNote$$aJournal Article
000877541 500__ $$aBiotechnologie 1
000877541 520__ $$aUnderstanding the composability of genetic elements is central to synthetic biology. Even for seemingly well-known elements such as a sigma 70 promoter the genetic context-dependent variability of promoter activity remains poorly understood. The lack of understanding of sequence to function results in highly limited de novo design of novel genetic element combinations. To address this issue, we characterized in detail concatenated “stacked” synthetic promoters including varying spacer sequence lengths and compared the transcription strength to the output of the individual promoters. The proxy for promoter activity, the msfGFP synthesis from stacked promoters was consistently lower than expected from the sum of the activities of the single promoters. While the spacer sequence itself had no activity, it drastically affected promoter activities when placed up- or downstream of a promoter. Single promoter-spacer combinations revealed a bivalent effect on msfGFP synthesis. By systematic analysis of promoter and spacer combinations, a semi-empirical correlation was developed to determine the combined activity of stacked promoters.
000877541 536__ $$0G:(DE-HGF)POF3-581$$a581 - Biotechnology (POF3-581)$$cPOF3-581$$fPOF III$$x0
000877541 588__ $$aDataset connected to CrossRef
000877541 7001_ $$0P:(DE-HGF)0$$aBlank, Lars M.$$b1
000877541 7001_ $$0P:(DE-Juel1)176653$$aWierckx, Nick$$b2$$eCorresponding author$$ufzj
000877541 773__ $$0PERI:(DE-600)2719493-0$$a10.3389/fbioe.2020.00551$$gVol. 8, p. 551$$p551$$tFrontiers in Bioengineering and Biotechnology$$v8$$x2296-4185$$y2020
000877541 8564_ $$uhttps://juser.fz-juelich.de/record/877541/files/2020-0251290-3.pdf
000877541 8564_ $$uhttps://juser.fz-juelich.de/record/877541/files/2020-0251290-3.pdf?subformat=pdfa$$xpdfa
000877541 8564_ $$uhttps://juser.fz-juelich.de/record/877541/files/K%C3%B6bbing%202020%20context%20effects%20on%20promoters.pdf$$yOpenAccess$$zStatID:(DE-HGF)0510
000877541 8564_ $$uhttps://juser.fz-juelich.de/record/877541/files/fbioe-08-00551.pdf$$yRestricted$$zStatID:(DE-HGF)0599
000877541 8564_ $$uhttps://juser.fz-juelich.de/record/877541/files/K%C3%B6bbing%202020%20context%20effects%20on%20promoters.pdf?subformat=pdfa$$xpdfa$$yOpenAccess$$zStatID:(DE-HGF)0510
000877541 8564_ $$uhttps://juser.fz-juelich.de/record/877541/files/fbioe-08-00551.pdf?subformat=pdfa$$xpdfa$$yRestricted$$zStatID:(DE-HGF)0599
000877541 8767_ $$82020-0251290-3$$92020-05-07$$d2020-06-15$$eAPC$$jDeposit$$lDeposit: Frontiers$$zUSD 2116.50
000877541 909CO $$ooai:juser.fz-juelich.de:877541$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire$$pdnbdelivery
000877541 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)176653$$aForschungszentrum Jülich$$b2$$kFZJ
000877541 9131_ $$0G:(DE-HGF)POF3-581$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vBiotechnology$$x0
000877541 9141_ $$y2020
000877541 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2020-01-14
000877541 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000877541 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bFRONT BIOENG BIOTECH : 2018$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000877541 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$f2020-01-14
000877541 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bFRONT BIOENG BIOTECH : 2018$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2020-01-14
000877541 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2020-01-14
000877541 920__ $$lyes
000877541 9201_ $$0I:(DE-Juel1)IBG-1-20101118$$kIBG-1$$lBiotechnologie$$x0
000877541 980__ $$ajournal
000877541 980__ $$aVDB
000877541 980__ $$aUNRESTRICTED
000877541 980__ $$aI:(DE-Juel1)IBG-1-20101118
000877541 980__ $$aAPC
000877541 9801_ $$aAPC
000877541 9801_ $$aFullTexts