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@ARTICLE{Nagel:877696,
      author       = {Nagel, Kerstin A. and Lenz, Henning and Kastenholz, Bernd
                      and Gilmer, Frank and Averesch, Andreas and Putz, Alexander
                      and Heinz, Kathrin and Fischbach, Andreas and Scharr, Hanno
                      and Fiorani, Fabio and Walter, Achim and Schurr, Ulrich},
      title        = {{T}he platform {G}row{S}creen-{A}gar enables identification
                      of phenotypic diversity in root and shoot growth traits of
                      agar grown plants},
      journal      = {Plant methods},
      volume       = {16},
      number       = {1},
      issn         = {1746-4811},
      address      = {London},
      publisher    = {BioMed Central},
      reportid     = {FZJ-2020-02406},
      pages        = {89},
      year         = {2020},
      abstract     = {BackgroundRoot system architecture and especially its
                      plasticity in acclimation to variable environments play a
                      crucial role in the ability of plants to explore and acquire
                      efficiently soil resources and ensure plant productivity.
                      Non-destructive measurement methods are indispensable to
                      quantify dynamic growth traits. For closing the phenotyping
                      gap, we have developed an automated phenotyping platform,
                      GrowScreen-Agar, for non-destructive characterization of
                      root and shoot traits of plants grown in transparent agar
                      medium.ResultsThe phenotyping system is capable to phenotype
                      root systems and correlate them to whole plant development
                      of up to 280 Arabidopsis plants within 15 min. The potential
                      of the platform has been demonstrated by quantifying
                      phenotypic differences within 78 Arabidopsis accessions from
                      the 1001 genomes project. The chosen concept
                      ‘plant-to-sensor’ is based on transporting plants to the
                      imaging position, which allows for flexible experimental
                      size and design. As transporting causes mechanical
                      vibrations of plants, we have validated that daily imaging,
                      and consequently, moving plants has negligible influence on
                      plant development. Plants are cultivated in square Petri
                      dishes modified to allow the shoot to grow in the ambient
                      air while the roots grow inside the Petri dish filled with
                      agar. Because it is common practice in the scientific
                      community to grow Arabidopsis plants completely enclosed in
                      Petri dishes, we compared development of plants that had the
                      shoot inside with that of plants that had the shoot outside
                      the plate. Roots of plants grown completely inside the Petri
                      dish grew $58\%$ slower, produced a 1.8 times higher lateral
                      root density and showed an etiolated shoot whereas plants
                      whose shoot grew outside the plate formed a rosette. In
                      addition, the setup with the shoot growing outside the plate
                      offers the unique option to accurately measure both, leaf
                      and root traits, non-destructively, and treat roots and
                      shoots separately.ConclusionsBecause the GrowScreen-Agar
                      system can be moved from one growth chamber to another,
                      plants can be phenotyped under a wide range of environmental
                      conditions including future climate scenarios. In
                      combination with a measurement throughput enabling
                      phenotyping a large set of mutants or accessions, the
                      platform will contribute to the identification of key
                      genes.},
      cin          = {IBG-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32582364},
      UT           = {WOS:000544856000001},
      doi          = {10.1186/s13007-020-00631-3},
      url          = {https://juser.fz-juelich.de/record/877696},
}