TY  - JOUR
AU  - Jäger, Vera D.
AU  - Lamm, Robin
AU  - Küsters, Kira
AU  - Ölçücü, Gizem
AU  - Oldiges, Marco
AU  - Jaeger, Karl-Erich
AU  - Büchs, Jochen
AU  - Krauss, Ulrich
TI  - Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application
JO  - Applied microbiology and biotechnology
VL  - 104
SN  - 0171-1741
CY  - New York
PB  - Springer
M1  - FZJ-2020-02480
SP  - 7313-7329
PY  - 2020
AB  - Bacterial inclusion bodies (IBs) have long been considered as inactive, unfolded waste material produced by heterologous overexpression of recombinant genes. In industrial applications, they are occasionally used as an alternative in cases where a protein cannot be expressed in soluble form and in high enough amounts. Then, however, refolding approaches are needed to transform inactive IBs into active soluble protein. While anecdotal reports about IBs themselves showing catalytic functionality/activity (CatIB) are found throughout literature, only recently, the use of protein engineering methods has facilitated the on-demand production of CatIBs. CatIB formation is induced usually by fusing short peptide tags or aggregation-inducing protein domains to a target protein. The resulting proteinaceous particles formed by heterologous expression of the respective genes can be regarded as a biologically produced bionanomaterial or, if enzymes are used as target protein, carrier-free enzyme immobilizates. In the present contribution, we review general concepts important for CatIB production, processing, and application.
LB  - PUB:(DE-HGF)16
C6  - pmid:32651598
UR  - <Go to ISI:>//WOS:000547221600005
DO  - DOI:10.1007/s00253-020-10760-3
UR  - https://juser.fz-juelich.de/record/877859
ER  -