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@ARTICLE{Jger:877859,
      author       = {Jäger, Vera D. and Lamm, Robin and Küsters, Kira and
                      Ölçücü, Gizem and Oldiges, Marco and Jaeger, Karl-Erich
                      and Büchs, Jochen and Krauss, Ulrich},
      title        = {{C}atalytically-active inclusion bodies for
                      biotechnology—general concepts, optimization, and
                      application},
      journal      = {Applied microbiology and biotechnology},
      volume       = {104},
      issn         = {0171-1741},
      address      = {New York},
      publisher    = {Springer},
      reportid     = {FZJ-2020-02480},
      pages        = {7313-7329},
      year         = {2020},
      abstract     = {Bacterial inclusion bodies (IBs) have long been considered
                      as inactive, unfolded waste material produced by
                      heterologous overexpression of recombinant genes. In
                      industrial applications, they are occasionally used as an
                      alternative in cases where a protein cannot be expressed in
                      soluble form and in high enough amounts. Then, however,
                      refolding approaches are needed to transform inactive IBs
                      into active soluble protein. While anecdotal reports about
                      IBs themselves showing catalytic functionality/activity
                      (CatIB) are found throughout literature, only recently, the
                      use of protein engineering methods has facilitated the
                      on-demand production of CatIBs. CatIB formation is induced
                      usually by fusing short peptide tags or aggregation-inducing
                      protein domains to a target protein. The resulting
                      proteinaceous particles formed by heterologous expression of
                      the respective genes can be regarded as a biologically
                      produced bionanomaterial or, if enzymes are used as target
                      protein, carrier-free enzyme immobilizates. In the present
                      contribution, we review general concepts important for CatIB
                      production, processing, and application.},
      cin          = {IBG-1 / IMET},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IMET-20090612},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32651598},
      UT           = {WOS:000547221600005},
      doi          = {10.1007/s00253-020-10760-3},
      url          = {https://juser.fz-juelich.de/record/877859},
}