TY  - JOUR
AU  - Beckers, Maximilian
AU  - Sachse, Carsten
TI  - Permutation testing of Fourier shell correlation for resolution estimation of cryo-EM maps
JO  - Journal of structural biology
VL  - 212
IS  - 1
SN  - 1047-8477
CY  - San Diego, Calif.
PB  - Elsevier
M1  - FZJ-2020-02607
SP  - 107579 -
PY  - 2020
AB  - Fourier shell correlation (FSC) has become a standard quantity for resolution estimation in electron cryo-microscopy. However, the resolution determination step is still subjective and not fully automated as it involves a series of map interventions before FSC computation and includes the selection of a common threshold. Here, we apply the statistical methods of permutation testing and false discovery rate (FDR) control to the resolution-dependent correlation measure. The approach allows fully automated and mask-free resolution determination based on statistical thresholding of FSC curves. We demonstrate the applicability for global, local and directional resolution estimation and show that the developed criterion termed FDR-FSC gives realistic resolution estimates based on statistical significance while eliminating the need of any map manipulations. The algorithms are implemented in a user-friendly GUI based software tool termed SPoC (https://github.com/MaximilianBeckers/SPOC).
LB  - PUB:(DE-HGF)16
C6  - pmid:32693019
UR  - <Go to ISI:>//WOS:000574936400006
DO  - DOI:10.1016/j.jsb.2020.107579
UR  - https://juser.fz-juelich.de/record/878063
ER  -