TY  - JOUR
AU  - Rosenbauer, Jakob
AU  - Zhang, Chengting
AU  - Mattes, Benjamin
AU  - Reinartz, Ines
AU  - Wedgwood, Kyle
AU  - Schindler, Simone
AU  - Sinner, Claude
AU  - Scholpp, Steffen
AU  - Schug, Alexander
TI  - Modeling of Wnt-mediated tissue patterning in vertebrate embryogenesis
JO  - PLoS Computational Biology
VL  - 16
IS  - 6
SN  - 1553-7358
CY  - San Francisco, Calif.
PB  - Public Library of Science
M1  - FZJ-2020-02612
SP  - e1007417 -
PY  - 2020
AB  - During embryogenesis, morphogens form a concentration gradient in responsive tissue, which is then translated into a spatial cellular pattern. The mechanisms by which morphogens spread through a tissue to establish such a morphogenetic field remain elusive. Here, we investigate by mutually complementary simulations and in vivo experiments how Wnt morphogen transport by cytonemes differs from typically assumed diffusion-based transport for patterning of highly dynamic tissue such as the neural plate in zebrafish. Stochasticity strongly influences fate acquisition at the single cell level and results in fluctuating boundaries between pattern regions. Stable patterning can be achieved by sorting through concentration dependent cell migration and apoptosis, independent of the morphogen transport mechanism. We show that Wnt transport by cytonemes achieves distinct Wnt thresholds for the brain primordia earlier compared with diffusion-based transport. We conclude that a cytoneme-mediated morphogen transport together with directed cell sorting is a potentially favored mechanism to establish morphogen gradients in rapidly expanding developmental systems.
LB  - PUB:(DE-HGF)16
C6  - pmid:32579554
UR  - <Go to ISI:>//WOS:000558077600003
DO  - DOI:10.1371/journal.pcbi.1007417
UR  - https://juser.fz-juelich.de/record/878070
ER  -