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@ARTICLE{Rosenbauer:878070,
author = {Rosenbauer, Jakob and Zhang, Chengting and Mattes, Benjamin
and Reinartz, Ines and Wedgwood, Kyle and Schindler, Simone
and Sinner, Claude and Scholpp, Steffen and Schug,
Alexander},
title = {{M}odeling of {W}nt-mediated tissue patterning in
vertebrate embryogenesis},
journal = {PLoS Computational Biology},
volume = {16},
number = {6},
issn = {1553-7358},
address = {San Francisco, Calif.},
publisher = {Public Library of Science},
reportid = {FZJ-2020-02612},
pages = {e1007417 -},
year = {2020},
abstract = {During embryogenesis, morphogens form a concentration
gradient in responsive tissue, which is then translated into
a spatial cellular pattern. The mechanisms by which
morphogens spread through a tissue to establish such a
morphogenetic field remain elusive. Here, we investigate by
mutually complementary simulations and in vivo experiments
how Wnt morphogen transport by cytonemes differs from
typically assumed diffusion-based transport for patterning
of highly dynamic tissue such as the neural plate in
zebrafish. Stochasticity strongly influences fate
acquisition at the single cell level and results in
fluctuating boundaries between pattern regions. Stable
patterning can be achieved by sorting through concentration
dependent cell migration and apoptosis, independent of the
morphogen transport mechanism. We show that Wnt transport by
cytonemes achieves distinct Wnt thresholds for the brain
primordia earlier compared with diffusion-based transport.
We conclude that a cytoneme-mediated morphogen transport
together with directed cell sorting is a potentially favored
mechanism to establish morphogen gradients in rapidly
expanding developmental systems.},
cin = {JSC / NIC},
ddc = {610},
cid = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)NIC-20090406},
pnm = {511 - Computational Science and Mathematical Methods
(POF3-511) / Forschergruppe Schug $(hkf6_20170901)$ / PhD no
Grant - Doktorand ohne besondere Förderung
(PHD-NO-GRANT-20170405)},
pid = {G:(DE-HGF)POF3-511 / $G:(DE-Juel1)hkf6_20170901$ /
G:(DE-Juel1)PHD-NO-GRANT-20170405},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32579554},
UT = {WOS:000558077600003},
doi = {10.1371/journal.pcbi.1007417},
url = {https://juser.fz-juelich.de/record/878070},
}