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@ARTICLE{Khawaja:878079,
      author       = {Khawaja, Anas and Itoh, Yuzuru and Remes, Cristina and
                      Spåhr, Henrik and Yukhnovets, Olessya and Höfig, Henning
                      and Amunts, Alexey and Rorbach, Joanna},
      title        = {{D}istinct pre-initiation steps in human mitochondrial
                      translation},
      journal      = {Nature Communications},
      volume       = {11},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {FZJ-2020-02621},
      pages        = {2932},
      year         = {2020},
      abstract     = {Translation initiation in human mitochondria relies upon
                      specialized mitoribosomes and initiation factors, mtIF2 and
                      mtIF3, which have diverged from their bacterial
                      counterparts. Here we report two distinct mitochondrial
                      pre-initiation assembly steps involving those factors.
                      Single-particle cryo-EM revealed that in the first step,
                      interactions between mitochondria-specific protein mS37 and
                      mtIF3 keep the small mitoribosomal subunit in a conformation
                      favorable for a subsequent accommodation of mtIF2 in the
                      second step. Combination with fluorescence cross-correlation
                      spectroscopy analyses suggests that mtIF3 promotes complex
                      assembly without mRNA or initiator tRNA binding, where
                      exclusion is achieved by the N-terminal and C-terminal
                      domains of mtIF3. Finally, the association of large
                      mitoribosomal subunit is required for initiator tRNA and
                      leaderless mRNA recruitment to form a stable initiation
                      complex. These data reveal fundamental aspects of mammal},
      cin          = {IBI-6},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBI-6-20200312},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32522994},
      UT           = {WOS:000543969100003},
      doi          = {10.1038/s41467-020-16503-2},
      url          = {https://juser.fz-juelich.de/record/878079},
}