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000878105 1001_ $$0P:(DE-HGF)0$$aCapo, Alessandro$$b0
000878105 245__ $$aStructural features of the glutamate-binding protein from Corynebacterium glutamicum
000878105 260__ $$aNew York, NY [u.a.]$$bElsevier$$c2020
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000878105 520__ $$aL-glutamate (Glu) is the major excitatory transmitter in mammalian brain. Inadequate concentration of Glu in the brain correlates to mood disorder. In industry, Glu is used as a flavour enhancer in food and in foodstuff processing. A high concentration of Glu has several effects on human health such as hypersensitive effects, headache and stomach pain. The presence of Glu in food can be detected by different analytical methods based on chromatography, or capillary electrophoresis or amperometric techniques. We have isolated and characterized a glutamate-binding protein (GluB) from the Gram-positive bacteria Corynebacterium glutamicum. Together with GluC protein, GluD protein and the cytoplasmic protein GluA, GluB permits the transport of Glu in/out of cell.In this study, we have investigated the binding features of GluB as well as the effect of temperature on its structure both in the absence and in the presence of Glu. The results have showed that GluB has a high affinity and selectivity versus Glu (nanomolar range) and the presence of the ligand induces a higher thermal stability of the protein structure.
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000878105 7001_ $$00000-0002-1489-272X$$aNatalello, Antonino$$b1
000878105 7001_ $$0P:(DE-Juel1)144031$$aMarienhagen, Jan$$b2
000878105 7001_ $$0P:(DE-HGF)0$$aPennacchio, Angela$$b3
000878105 7001_ $$0P:(DE-HGF)0$$aCamarca, Alessandra$$b4
000878105 7001_ $$0P:(DE-HGF)0$$aDi Giovanni, Stefano$$b5
000878105 7001_ $$0P:(DE-HGF)0$$aStaiano, Maria$$b6
000878105 7001_ $$0P:(DE-HGF)0$$aD'Auria, Sabato$$b7$$eCorresponding author
000878105 7001_ $$0P:(DE-HGF)0$$aVarriale, Antonio$$b8
000878105 773__ $$0PERI:(DE-600)1483284-7$$a10.1016/j.ijbiomac.2020.06.197$$gVol. 162, p. 903 - 912$$p903 - 912$$tInternational journal of biological macromolecules$$v162$$x0141-8130$$y2020
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