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@ARTICLE{Rollenhagen:878138,
      author       = {Rollenhagen, Astrid and Walkenfort, Bernd and Yakoubi,
                      Rachida and Klauke, Sarah A. and Schmuhl-Giesen, Sandra F.
                      and Heinen-Weiler, Jacqueline and Voortmann, Sylvia and
                      Marshallsay, Brigitte and Palaz, Tayfun and Holz, Ulrike and
                      Hasenberg, Mike and Lübke, Joachim H. R.},
      title        = {{S}ynaptic {O}rganization of the {H}uman {T}emporal {L}obe
                      {N}eocortex as {R}evealed by {H}igh-{R}esolution
                      {T}ransmission, {F}ocused {I}on {B}eam {S}canning, and
                      {E}lectron {M}icroscopic {T}omography},
      journal      = {International journal of molecular sciences},
      volume       = {21},
      number       = {15},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2020-02655},
      pages        = {5558 -},
      year         = {2020},
      abstract     = {Modern electron microscopy (EM) such as fine-scale
                      transmission EM, focused ion beam scanning EM, and EM
                      tomography have enormously improved our knowledge about the
                      synaptic organization of the normal, developmental, and
                      pathologically altered brain. In contrast to various animal
                      species, comparably little is known about these structures
                      in the human brain. Non-epileptic neocortical access tissue
                      from epilepsy surgery was used to generate quantitative 3D
                      models of synapses. Beside the overall geometry, the number,
                      size, and shape of active zones and of the three
                      functionally defined pools of synaptic vesicles representing
                      morphological correlates for synaptic transmission and
                      plasticity were quantified. EM tomography further allowed
                      new insights in the morphological organization and size of
                      the functionally defined readily releasable pool. Beside
                      similarities, human synaptic boutons, although comparably
                      small (approximately 5 µm), differed substantially in
                      several structural parameters, such as the shape and size of
                      active zones, which were on average 2 to 3-fold larger than
                      in experimental animals. The total pool of synaptic vesicles
                      exceeded that in experimental animals by approximately 2 to
                      3-fold, in particular the readily releasable and recycling
                      pool by approximately 2 to 5-fold, although these pools
                      seemed to be layer-specifically organized. Taken together,
                      synaptic boutons in the human temporal lobe neocortex
                      represent unique entities perfectly adapted to the “job”
                      they have to fulfill in the circuitry in which they are
                      embedded. Furthermore, the quantitative 3D models of
                      synaptic boutons are useful to explain and even predict the
                      functional properties of synaptic connections in the human
                      neocortex.},
      cin          = {INM-10},
      ddc          = {540},
      cid          = {I:(DE-Juel1)INM-10-20170113},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32756507},
      UT           = {WOS:000559578200001},
      doi          = {10.3390/ijms21155558},
      url          = {https://juser.fz-juelich.de/record/878138},
}