% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Beckers:878277,
      author       = {Beckers, Maximilian and Mann, Daniel and Sachse, Carsten},
      title        = {{S}tructural interpretation of cryo-{EM} image
                      reconstructions},
      journal      = {Progress in biophysics $\&$ molecular biology},
      volume       = {160},
      issn         = {0079-6107},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2020-02740},
      pages        = {26-36},
      year         = {2021},
      abstract     = {The productivity of single-particle cryo-EM as a structure
                      determination method has rapidly increased as many novel
                      biological structures are being elucidated. The ultimate
                      result of the cryo-EM experiment is an atomic model that
                      should faithfully represent the computed image
                      reconstruction. Although the principal approach of atomic
                      model building and refinement from maps resembles that of
                      the X-ray crystallographic methods, there are important
                      differences due to the unique properties resulting from the
                      3D image reconstructions. In this review, we discuss the
                      practiced work-flow from the cryo-EM image reconstruction to
                      the atomic model. We give an overview of (i) resolution
                      determination methods in cryo-EM including local and
                      directional resolution variation, (ii) cryo-EM map contrast
                      optimization including complementary map types that can help
                      in identifying ambiguous density features, (iii) atomic
                      model building and (iv) refinement in various resolution
                      regimes including (v) their validation and (vi) discuss
                      differences between X-ray and cryo-EM maps. Based on the
                      methods originally developed for X-ray crystallography, the
                      path from 3D image reconstruction to atomic coordinates has
                      become an integral and important part of the cryo-EM
                      structure determination work-flow that routinely delivers
                      atomic models.},
      cin          = {ER-C-3},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ER-C-3-20170113},
      pnm          = {5352 - Understanding the Functionality of Soft Matter and
                      Biomolecular Systems (POF4-535) / 5241 - Molecular
                      Information Processing in Cellular Systems (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32735944},
      UT           = {WOS:000632013800005},
      doi          = {10.1016/j.pbiomolbio.2020.07.004},
      url          = {https://juser.fz-juelich.de/record/878277},
}