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@ARTICLE{Pfaff:878346,
author = {Pfaff, Julia and Denton, Alisandra K. and Usadel, Björn
and Pfaff, Christian},
title = {{P}hosphate starvation causes different stress responses in
the lipid metabolism of tomato leaves and roots},
journal = {Biochimica et biophysica acta / Molecular and cell biology
of lipids Molecular and cell biology of lipids},
volume = {1865},
number = {9},
issn = {1388-1981},
address = {Amsterdam},
publisher = {Elsevier},
reportid = {FZJ-2020-02794},
pages = {158763 -},
year = {2020},
abstract = {Plants have evolved various acclimation responses to cope
with phosphate depletion, including several changes in lipid
metabolism. Thereby membrane phospholipids are
dephosphorylated and can be used as an internal phosphate
source, while galactolipids are incorporated into the
membrane to maintain membrane functionality. Still little is
known about the lipidomic and transcriptomic response of
plants other than Arabidopsis thaliana upon phosphate
starvation. Therefore, we employed lipidomics and
transcriptomics to characterize the phosphate starvation
response of lipid metabolism in tomato leaves and
roots.Overall, phospholipid levels decreased and
galactolipids increased during the acclimation response. In
addition, an early increase of triacylglycerol was observed.
Interestingly, there were major differences in the
acclimation response of tomato leaves and roots: leaves
mainly accumulated polyunsaturated triacylglycerol, while
roots showed a massive increase in galactolipid content. In
line with these results, we observed transcriptional
induction of phospholipid degradation and galactolipid
synthesis pathways in both analyzed tissues. In contrast,
other aspects of the transcriptional response, in
particular, the induction of phospholipid degradation,
ER-localized fatty acid desaturation and triacylglycerol
assembly differed between tomato leaves and roots.These
results suggest a different modulation of degraded
phospholipids toward triacylglycerols and galactolipids in
phosphate-starved tomato leaves and roots. Possibly the
availability and composition of acyl-CoA pools and
ER-derived precursors trigger the synthesis of
triacylglycerols or galactolipids. As the mechanism of
triacylglycerol accumulation is poorly characterized outside
of seed oil formation, these findings enhance our
understanding of the phosphate starvation response and of
how storage lipids accumulate under stress in vegetative
tissue. Previous article in issue},
cin = {IBG-4},
ddc = {570},
cid = {I:(DE-Juel1)IBG-4-20200403},
pnm = {582 - Plant Science (POF3-582) / 583 - Innovative
Synergisms (POF3-583)},
pid = {G:(DE-HGF)POF3-582 / G:(DE-HGF)POF3-583},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32645408},
UT = {WOS:000552710900023},
doi = {10.1016/j.bbalip.2020.158763},
url = {https://juser.fz-juelich.de/record/878346},
}