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@ARTICLE{Skra:878492,
      author       = {Skóra, Tomasz and Vaghefikia, Farzaneh and Fitter, Jörg
                      and Kondrat, Svyatoslav},
      title        = {{M}acromolecular {C}rowding: {H}ow {S}hape and
                      {I}nteractions {A}ffect {D}iffusion},
      journal      = {The journal of physical chemistry / B},
      volume       = {124},
      number       = {35},
      issn         = {1520-5207},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {FZJ-2020-02881},
      pages        = {7537–7543},
      year         = {2020},
      abstract     = {A significant fraction of the cell volume is occupied by
                      various proteins, polysaccharides, nucleic acids, etc.,
                      which considerably reduces the mobility of macromolecules.
                      Theoretical and experimental work so far have mainly focused
                      on the dependence of the mobility on the occupied volume,
                      while the effect of a macromolecular shape received less
                      attention. Herein, using fluorescence correlation
                      spectroscopy (FCS) and Brownian dynamics (BD) simulations,
                      we report on a dramatic slowdown of tracer diffusion by
                      cylindrically shaped double-stranded (ds) DNAs (16 nm in
                      length). We find, for instance, that the translational
                      diffusion coefficient of a streptavidin tracer is reduced by
                      about $60\%$ for a volume fraction of dsDNA as low as just
                      $5\%.$ For comparison, for a spherical crowder (Ficoll70)
                      the slowdown is only $10\%$ at the same volume fraction and
                      $60\%$ reduction occurs at a volume fraction as high as
                      $35\%.$ BD simulations reveal that this reduction can be
                      attributed to a larger volume excluded to a tracer by dsDNA
                      particles, as compared with spherical Ficoll70 at the same
                      volume fraction, and to the differences in the
                      tracer–crowder attractive interactions. In addition, we
                      find using BD simulations that rotational diffusion of dsDNA
                      is less affected by the crowder shape than its translational
                      motion. Our results show that diffusion in crowded systems
                      is determined not merely by the occupied volume fraction,
                      but that the shape and interactions can determine diffusion,
                      which is relevant to the diverse intracellular environments
                      inside living cells.},
      cin          = {IBI-6},
      ddc          = {530},
      cid          = {I:(DE-Juel1)IBI-6-20200312},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32790396},
      UT           = {WOS:000569371200002},
      doi          = {10.1021/acs.jpcb.0c04846},
      url          = {https://juser.fz-juelich.de/record/878492},
}