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@ARTICLE{vanderMeer:878576,
      author       = {van der Meer, A.-M. and Berger, T. and Müller, Frank and
                      Foldenauer, A. C. and Johnen, S. and Walter, P.},
      title        = {{E}stablishment and characterization of a unilateral
                      {UV}-induced photoreceptor degeneration model in the
                      {C}57{BL}/6{J} mouse},
      journal      = {Translational Vision Science $\&$ Technology},
      volume       = {9},
      number       = {9},
      issn         = {2164-2591},
      address      = {Rockville, Md.},
      publisher    = {ARVO},
      reportid     = {FZJ-2020-02919},
      pages        = {21},
      year         = {2020},
      abstract     = {Purpose: To investigate whether UV irradiation of the mouse
                      eye can induce photoreceptor degeneration, producing a
                      phenotype reminiscent of the rd10 mouse, left eyes of female
                      C57Bl/6J mice were irradiated with a UV LED array
                      (370 nm). A lens was placed between the cornea and LED,
                      allowing illumination of about one-third of the retina. The
                      short-term and long-term effects on the retina were
                      evaluated.Methods: First, a dose escalation study, in which
                      corneal dosages between 2.8 and 9.3 J/cm2 were tested, was
                      performed. A dosage of 7.5 J/cm2 was chosen for the
                      following characterization study. Before and after
                      irradiation slit-lamp examinations, full-field
                      electroretinography, spectral domain optical coherence
                      tomography and macroscopy were performed. After different
                      time spans (5 days to 12 weeks) the animals were sacrificed
                      and the retinae used for immunohistochemistry or
                      multielectrode array testing. Right eyes served as untreated
                      controls.Results: In treated eyes, spectral domain optical
                      coherence tomography revealed a decrease in retinal
                      thickness to $53\%.$ Full-field electroretinography
                      responses decreased significantly from day 5 on in treated
                      eyes. Multielectrode array recordings revealed oscillatory
                      potentials with a mean frequency of 5.2 ±0.6 Hz in the
                      illuminated area. Structural changes in the retina were
                      observed in immunohistochemical staining.Conclusions: UV
                      irradiation proved to be efficient in inducing photoreceptor
                      degeneration in the mouse retina, while leaving the other
                      retinal layers largely intact. The irradiated area of
                      treated eyes can be identified easily in spectral domain
                      optical coherence tomography and in explanted
                      retinae.Translational Relevance: This study provides
                      information on anatomic and functional changes in UV-treated
                      retina, enabling the use of this model for retinitis
                      pigmentosa-like diseases in animals suited for experimental
                      retinal surgery.},
      cin          = {IBI-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-1-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32879777},
      UT           = {WOS:000582929100021},
      doi          = {10.1167/tvst.9.9.21},
      url          = {https://juser.fz-juelich.de/record/878576},
}