TY  - JOUR
AU  - Otto, Maike
AU  - Wynands, Benedikt
AU  - Marienhagen, Jan
AU  - Blank, Lars M.
AU  - Wierckx, Nick
TI  - Benzoate Synthesis from Glucose or Glycerol Using Engineered Pseudomonas taiwanensis
JO  - Biotechnology journal
VL  - 15
IS  - 11
SN  - 1860-7314
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2020-03031
SP  - 2000211 -
PY  - 2020
N1  - weitere Grants: German Research Foundation (DFG) through the Emmy Noether program (WI 4255/1‐1) German Federal Ministry of Education and Research (BMBF) through the NO‐STRESS project (FKZ 031B0852A)
AB  - Benzoic acid is one of the most commonly used food preservatives, but currently exclusively produced in petrochemical processes. In this study, a bio‐based production pathway using an engineered strain of Pseudomonas taiwanensis is described. In a phenylalanine‐overproducing strain, bacterial and plant genes are heterologously expressed to achieve production of benzoate via a β‐oxidation pathway. Strategic disruption of the native Pseudomonas benzoate degradation pathway further allows the production of catechol and cis,cis‐muconate. Taken together, this work demonstrates new routes for the microbial production of these industrially relevant chemicals from renewable resources.
LB  - PUB:(DE-HGF)16
C6  - pmid:32721071
UR  - <Go to ISI:>//WOS:000567363700001
DO  - DOI:10.1002/biot.202000211
UR  - https://juser.fz-juelich.de/record/878731
ER  -