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000884059 1001_ $$00000-0001-9246-6673$$aSiewert, Christian D.$$b0
000884059 245__ $$aHybrid Biopolymer and Lipid Nanoparticles with Improved Transfection Efficacy for mRNA
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000884059 520__ $$aHybrid nanoparticles from lipidic and polymeric components were assembled to serve as vehicles for the transfection of messenger RNA (mRNA) using different portions of the cationic lipid DOTAP (1,2-Dioleoyl-3-trimethylammonium-propane) and the cationic biopolymer protamine as model systems. Two different sequential assembly approaches in comparison with a direct single-step protocol were applied, and molecular organization in correlation with biological activity of the resulting nanoparticle systems was investigated. Differences in the structure of the nanoparticles were revealed by thorough physicochemical characterization including small angle neutron scattering (SANS), small angle X-ray scattering (SAXS), and cryogenic transmission electron microscopy (cryo-TEM). All hybrid systems, combining lipid and polymer, displayed significantly increased transfection in comparison to lipid/mRNA and polymer/mRNA particles alone. For the hybrid nanoparticles, characteristic differences regarding the internal organization, release characteristics, and activity were determined depending on the assembly route. The systems with the highest transfection efficacy were characterized by a heterogenous internal organization, accompanied by facilitated release. Such a system could be best obtained by the single step protocol, starting with a lipid and polymer mixture for nanoparticle formation
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000884059 65017 $$0V:(DE-MLZ)GC-1602-2016$$2V:(DE-HGF)$$aPolymers, Soft Nano Particles and  Proteins$$x0
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000884059 7001_ $$00000-0002-5517-5970$$aHaas, Heinrich$$b1
000884059 7001_ $$0P:(DE-HGF)0$$aCornet, Vera$$b2
000884059 7001_ $$0P:(DE-HGF)0$$aNogueira, Sara S.$$b3
000884059 7001_ $$0P:(DE-HGF)0$$aNawroth, Thomas$$b4
000884059 7001_ $$00000-0003-0568-9540$$aUebbing, Lukas$$b5
000884059 7001_ $$0P:(DE-HGF)0$$aZiller, Antje$$b6
000884059 7001_ $$0P:(DE-HGF)0$$aAl-Gousous, Jozef$$b7
000884059 7001_ $$0P:(DE-Juel1)130905$$aRadulescu, Aurel$$b8
000884059 7001_ $$00000-0002-0747-3965$$aSchroer, Martin A.$$b9
000884059 7001_ $$0P:(DE-HGF)0$$aBlanchet, Clement E.$$b10
000884059 7001_ $$0P:(DE-HGF)0$$aSvergun, Dmitri I.$$b11
000884059 7001_ $$00000-0002-3991-5721$$aRadsak, Markus P.$$b12
000884059 7001_ $$0P:(DE-HGF)0$$aSahin, Ugur$$b13
000884059 7001_ $$0P:(DE-HGF)0$$aLangguth, Peter$$b14$$eCorresponding author
000884059 773__ $$0PERI:(DE-600)2661518-6$$a10.3390/cells9092034$$gVol. 9, no. 9, p. 2034 -$$n9$$p2034$$tCells$$v9$$x2073-4409$$y2020
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