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@ARTICLE{Siewert:884059,
      author       = {Siewert, Christian D. and Haas, Heinrich and Cornet, Vera
                      and Nogueira, Sara S. and Nawroth, Thomas and Uebbing, Lukas
                      and Ziller, Antje and Al-Gousous, Jozef and Radulescu, Aurel
                      and Schroer, Martin A. and Blanchet, Clement E. and Svergun,
                      Dmitri I. and Radsak, Markus P. and Sahin, Ugur and
                      Langguth, Peter},
      title        = {{H}ybrid {B}iopolymer and {L}ipid {N}anoparticles with
                      {I}mproved {T}ransfection {E}fficacy for m{RNA}},
      journal      = {Cells},
      volume       = {9},
      number       = {9},
      issn         = {2073-4409},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2020-03070},
      pages        = {2034},
      year         = {2020},
      abstract     = {Hybrid nanoparticles from lipidic and polymeric components
                      were assembled to serve as vehicles for the transfection of
                      messenger RNA (mRNA) using different portions of the
                      cationic lipid DOTAP
                      (1,2-Dioleoyl-3-trimethylammonium-propane) and the cationic
                      biopolymer protamine as model systems. Two different
                      sequential assembly approaches in comparison with a direct
                      single-step protocol were applied, and molecular
                      organization in correlation with biological activity of the
                      resulting nanoparticle systems was investigated. Differences
                      in the structure of the nanoparticles were revealed by
                      thorough physicochemical characterization including small
                      angle neutron scattering (SANS), small angle X-ray
                      scattering (SAXS), and cryogenic transmission electron
                      microscopy (cryo-TEM). All hybrid systems, combining lipid
                      and polymer, displayed significantly increased transfection
                      in comparison to lipid/mRNA and polymer/mRNA particles
                      alone. For the hybrid nanoparticles, characteristic
                      differences regarding the internal organization, release
                      characteristics, and activity were determined depending on
                      the assembly route. The systems with the highest
                      transfection efficacy were characterized by a heterogenous
                      internal organization, accompanied by facilitated release.
                      Such a system could be best obtained by the single step
                      protocol, starting with a lipid and polymer mixture for
                      nanoparticle formation},
      cin          = {JCNS-FRM-II / JCNS-1 / MLZ},
      ddc          = {570},
      cid          = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
                      I:(DE-Juel1)JCNS-1-20110106 / I:(DE-588b)4597118-3},
      pnm          = {6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
                      / 6G15 - FRM II / MLZ (POF3-6G15)},
      pid          = {G:(DE-HGF)POF3-6G4 / G:(DE-HGF)POF3-6G15},
      experiment   = {EXP:(DE-MLZ)KWS2-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32899484},
      UT           = {WOS:000580699400001},
      doi          = {10.3390/cells9092034},
      url          = {https://juser.fz-juelich.de/record/884059},
}