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@ARTICLE{Hemmerich:884115,
      author       = {Hemmerich, Johannes and Labib, Mohamed and Steffens, Carmen
                      and Reich, Sebastian J. and Weiske, Marc and Baumgart, Meike
                      and Rückert, Christian and Ruwe, Matthias and Siebert,
                      Daniel and Wendisch, Volker F. and Kalinowski, Jörn and
                      Wiechert, Wolfgang and Oldiges, Marco},
      title        = {{S}creening of a genome-reduced {C}orynebacterium
                      glutamicum strain library for improved heterologous cutinase
                      secretion},
      journal      = {Microbial biotechnology},
      volume       = {13},
      number       = {6},
      issn         = {1751-7915},
      address      = {Oxford},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2020-03102},
      pages        = {2020-2031},
      year         = {2020},
      abstract     = {The construction of microbial platform organisms by means
                      of genome reduction is an ongoing topic in biotechnology. In
                      this study, we investigated whether the deletion of single
                      or multiple gene clusters has a positive effect on the
                      secretion of cutinase from Fusarium solani pisi in the
                      industrial workhorse Corynebacterium glutamicum. A total of
                      22 genome‐reduced strain variants were compared applying
                      two Sec signal peptides from Bacillus subtilis.
                      High‐throughput phenotyping using robotics‐integrated
                      microbioreactor technology with automated harvesting
                      revealed distinct cutinase secretion performance for a
                      specific combination of signal peptide and genomic
                      deletions. The biomass‐specific cutinase yield for strain
                      $GRS41_51_NprE$ was increased by ~ $200\%,$ although the
                      growth rate was reduced by ~ $60\%.$ Importantly, the
                      causative deletions of genomic clusters cg2801‐cg2828 and
                      rrnC‐cg3298 could not have been inferred a priori.
                      Strikingly, bioreactor fed‐batch cultivations at
                      controlled growth rates resulted in a complete reversal of
                      the screening results, with the cutinase yield for strain
                      $GRS41_51_NprE$ dropping by ~ $25\%$ compared to the
                      reference strain. Thus, the choice of bioprocess conditions
                      may turn a ‘high‐performance’ strain from batch
                      screening into a ‘low‐performance’ strain in
                      fed‐batch cultivation. In conclusion, future studies are
                      needed in order to understand metabolic adaptations of C.
                      glutamicum to both genomic deletions and different
                      bioprocess conditions.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32893457},
      UT           = {WOS:000566379800001},
      doi          = {10.1111/1751-7915.13660},
      url          = {https://juser.fz-juelich.de/record/884115},
}