TY  - JOUR
AU  - Kubicki, Sonja
AU  - Bator, Isabel
AU  - Jankowski, Silke
AU  - Schipper, Kerstin
AU  - Tiso, Till
AU  - Feldbrügge, Michael
AU  - Blank, Lars M.
AU  - Thies, Stephan
AU  - Jaeger, Karl-Erich
TI  - A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants
JO  - Frontiers in Bioengineering and Biotechnology
VL  - 8
SN  - 2296-4185
CY  - Lausanne
PB  - Frontiers Media
M1  - FZJ-2020-03179
SP  - 958
PY  - 2020
AB  - A large variety of microorganisms produces biosurfactants with the potential for a number of diverse industrial applications. To identify suitable wild-type or engineered production strains, efficient screening methods are needed, allowing for rapid and reliable quantification of biosurfactants in multiple cultures, preferably at high throughput. To this end, we have established a novel and sensitive assay for the quantification of biosurfactants based on the dye Victoria Pure Blue BO (VPBO). The assay allows the colorimetric assessment of biosurfactants directly in culture supernatants and does not require extraction or concentration procedures. Working ranges were determined for precise quantification of different rhamnolipid biosurfactants; titers in culture supernatants of recombinant Pseudomonas putida KT2440 calculated by this assay were confirmed to be the same ranges detected by independent high-performance liquid chromatography (HPLC)-charged aerosol detector (CAD) analyses. The assay was successfully applied for detection of chemically different anionic or non-ionic biosurfactants including mono- and di-rhamnolipids (glycolipids), mannosylerythritol lipids (MELs, glycolipids), 3-(3-hydroxyalkanoyloxy) alkanoic acids (fatty acid conjugates), serrawettin W1 (lipopeptide), and N-acyltyrosine (lipoamino acid). In summary, the VPBO assay offers a broad range of applications including the comparative evaluation of different cultivation conditions and high-throughput screening of biosurfactant-producing microbial strains.
LB  - PUB:(DE-HGF)16
C6  - 32974305
UR  - <Go to ISI:>//WOS:000567817400001
DO  - DOI:10.3389/fbioe.2020.00958
UR  - https://juser.fz-juelich.de/record/884287
ER  -