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@ARTICLE{Kubicki:884287,
      author       = {Kubicki, Sonja and Bator, Isabel and Jankowski, Silke and
                      Schipper, Kerstin and Tiso, Till and Feldbrügge, Michael
                      and Blank, Lars M. and Thies, Stephan and Jaeger,
                      Karl-Erich},
      title        = {{A} {S}traightforward {A}ssay for {S}creening and
                      {Q}uantification of {B}iosurfactants in {M}icrobial
                      {C}ulture {S}upernatants},
      journal      = {Frontiers in Bioengineering and Biotechnology},
      volume       = {8},
      issn         = {2296-4185},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2020-03179},
      pages        = {958},
      year         = {2020},
      abstract     = {A large variety of microorganisms produces biosurfactants
                      with the potential for a number of diverse industrial
                      applications. To identify suitable wild-type or engineered
                      production strains, efficient screening methods are needed,
                      allowing for rapid and reliable quantification of
                      biosurfactants in multiple cultures, preferably at high
                      throughput. To this end, we have established a novel and
                      sensitive assay for the quantification of biosurfactants
                      based on the dye Victoria Pure Blue BO (VPBO). The assay
                      allows the colorimetric assessment of biosurfactants
                      directly in culture supernatants and does not require
                      extraction or concentration procedures. Working ranges were
                      determined for precise quantification of different
                      rhamnolipid biosurfactants; titers in culture supernatants
                      of recombinant Pseudomonas putida KT2440 calculated by this
                      assay were confirmed to be the same ranges detected by
                      independent high-performance liquid chromatography
                      (HPLC)-charged aerosol detector (CAD) analyses. The assay
                      was successfully applied for detection of chemically
                      different anionic or non-ionic biosurfactants including
                      mono- and di-rhamnolipids (glycolipids), mannosylerythritol
                      lipids (MELs, glycolipids), 3-(3-hydroxyalkanoyloxy)
                      alkanoic acids (fatty acid conjugates), serrawettin W1
                      (lipopeptide), and N-acyltyrosine (lipoamino acid). In
                      summary, the VPBO assay offers a broad range of applications
                      including the comparative evaluation of different
                      cultivation conditions and high-throughput screening of
                      biosurfactant-producing microbial strains.},
      cin          = {IBG-1 / IMET},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IMET-20090612},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32974305},
      UT           = {WOS:000567817400001},
      doi          = {10.3389/fbioe.2020.00958},
      url          = {https://juser.fz-juelich.de/record/884287},
}