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@ARTICLE{Wiechert:884748,
      author       = {Wiechert, Johanna and Gätgens, Cornelia and Wirtz, Astrid
                      and Frunzke, Julia},
      title        = {{I}nducible {E}xpression {S}ystems {B}ased on {X}enogeneic
                      {S}ilencing and {C}ounter-{S}ilencing and {D}esign of a
                      {M}etabolic {T}oggle {S}witch},
      journal      = {ACS synthetic biology},
      volume       = {9},
      number       = {8},
      issn         = {2161-5063},
      address      = {Washington, DC},
      publisher    = {ACS},
      reportid     = {FZJ-2020-03237},
      pages        = {2023 - 2038},
      year         = {2020},
      abstract     = {Inducible expression systems represent key modules in
                      regulatory circuit design and metabolic engineering
                      approaches. However, established systems are often limited
                      in terms of applications due to high background expression
                      levels and inducer toxicity. In bacteria, xenogeneic
                      silencing (XS) proteins are involved in the tight control of
                      horizontally acquired, AT-rich DNA. The action of XS
                      proteins may be opposed by interference with a specific
                      transcription factor, resulting in the phenomenon of
                      counter-silencing, thereby activating gene expression. In
                      this study, we harnessed this principle for the construction
                      of a synthetic promoter library consisting of phage
                      promoters targeted by the Lsr2-like XS protein CgpS of
                      Corynebacterium glutamicum. Counter-silencing was achieved
                      by inserting the operator sequence of the
                      gluconate-responsive transcription factor GntR. The
                      GntR-dependent promoter library is comprised of 28 activated
                      and 16 repressed regulatory elements featuring
                      effector-dependent tunability. For selected candidates,
                      background expression levels were confirmed to be
                      significantly reduced in comparison to established
                      heterologous expression systems. Finally, a GntR-dependent
                      metabolic toggle switch was implemented in a C.
                      glutamicuml-valine production strain allowing the dynamic
                      redirection of carbon flux between biomass and product
                      formation.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32649183},
      UT           = {WOS:000563758100010},
      doi          = {10.1021/acssynbio.0c00111},
      url          = {https://juser.fz-juelich.de/record/884748},
}