%0 Journal Article
%A Muschallik, Lukas
%A Kipp, Carina Ronja
%A Recker, Inga
%A Bongaerts, Johannes
%A Pohl, Martina
%A Gellissen, Melanie
%A Schöning, Michael J.
%A Selmer, Thorsten
%A Siegert, Petra
%T Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2,3-diol dehydrogenase
%J Journal of biotechnology
%V 324
%@ 0168-1656
%C Amsterdam [u.a.]
%I Elsevier Science
%M FZJ-2020-03833
%P 61 - 70
%D 2020
%X The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 32976868
%U <Go to ISI:>//WOS:000600955400007
%R 10.1016/j.jbiotec.2020.09.016
%U https://juser.fz-juelich.de/record/885450