TY  - JOUR
AU  - Muschallik, Lukas
AU  - Kipp, Carina Ronja
AU  - Recker, Inga
AU  - Bongaerts, Johannes
AU  - Pohl, Martina
AU  - Gellissen, Melanie
AU  - Schöning, Michael J.
AU  - Selmer, Thorsten
AU  - Siegert, Petra
TI  - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2,3-diol dehydrogenase
JO  - Journal of biotechnology
VL  - 324
SN  - 0168-1656
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2020-03833
SP  - 61 - 70
PY  - 2020
AB  - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.
LB  - PUB:(DE-HGF)16
C6  - 32976868
UR  - <Go to ISI:>//WOS:000600955400007
DO  - DOI:10.1016/j.jbiotec.2020.09.016
UR  - https://juser.fz-juelich.de/record/885450
ER  -