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@ARTICLE{Ohashi:885457,
author = {Ohashi, Takao and Fujisawa, Yu and Hayes, Marc and Misaki,
Ryo and Pietruszka, Jörg and Fujiyama, Kazuhito},
title = {{T}ransglycosylation toward naringenin-7-{O}-glucoside
using an {N}180{H} mutant of {C}oprinopsis cinerea
endo-β-{N}-acetylglucosaminidase},
journal = {Biochemical and biophysical research communications},
volume = {530},
number = {1},
issn = {0006-291X},
address = {Orlando, Fla.},
publisher = {Academic Press},
reportid = {FZJ-2020-03840},
pages = {155 - 159},
year = {2020},
abstract = {Flavonoids are generally glycosylated, and the glycan
moieties of flavonoid glycosides are known to greatly affect
their physicochemical and biological properties. Thus, the
development of a variety of tools for glycan remodeling of
flavonoid glycosides is highly desired. An
endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which
is developed as an excellent chemoenzymatic tool for
creating sialylglycoproteins, was employed for the
glycosylation of flavonoids. Endo-CC N180H transferred the
sialyl biantennary glycans from the sialylglyco peptide to
pNP-GlcNAc and narigenin-7-O-glucoside. The kinetic
parameters of Endo-CC N180H towards SGP and pNP-GlcNAc were
determined. Flavonoid glucosides harboring a 1,3-diol
structure in the glucose moieties acted as substrates of
Endo-CC N180H. We proposed that the sialyl biantennary
glycan transfer to the flavonoid by Endo-CC N180H could pave
the way for the improvement of the inherent biological
functions of the flavonoids and creation of novel flavonoid
glycoside derivatives for future human health benefits
including foods and drugs.},
cin = {IBOC / IBG-1},
ddc = {570},
cid = {I:(DE-Juel1)IBOC-20090406 / I:(DE-Juel1)IBG-1-20101118},
pnm = {581 - Biotechnology (POF3-581)},
pid = {G:(DE-HGF)POF3-581},
typ = {PUB:(DE-HGF)16},
pubmed = {32828279},
UT = {WOS:000565184300025},
doi = {10.1016/j.bbrc.2020.06.128},
url = {https://juser.fz-juelich.de/record/885457},
}