% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Hogenkamp:885458,
      author       = {Hogenkamp, Fabian and Hilgers, Fabienne and Knapp, Andreas
                      and Klaus, Oliver and Bier, Claus and Binder, Dennis and
                      Jaeger, Karl-Erich and Drepper, Thomas and Pietruszka,
                      Jörg},
      title        = {{E}ffect of {P}hotocaged {I}sopropyl
                      β‐d‐1‐{T}hiogalactopyranoside {S}olubility on
                      {L}ight‐{R}esponsiveness of {L}ac{I}‐controlled
                      {E}xpression {S}ystems in {D}ifferent {B}acteria},
      journal      = {ChemBioChem},
      volume       = {22},
      number       = {3},
      issn         = {1439-7633},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2020-03841},
      pages        = {539-547},
      year         = {2021},
      abstract     = {Photolabile protecting groups play a significant role in
                      controlling biological functions and cellular processes in
                      living cells and tissues, as light offers high
                      spatiotemporal control, is non‐invasive as well as easily
                      tuneable. In the recent past, photo‐responsive inducer
                      molecules such as 6‐nitropiperonyl‐caged IPTG
                      (NP‐cIPTG) have been used as optochemical tools for Lac
                      repressor‐controlled microbial expression systems. To
                      further expand the applicability of the versatile
                      optochemical on‐switch, we have investigated whether the
                      modulation of cIPTG water solubility can improve the light
                      responsiveness of appropriate expression systems in
                      bacteria. To this end, we developed two new cIPTG
                      derivatives with different hydrophobicity and demonstrated
                      both an easy applicability for the light‐mediated control
                      of gene expression and a simple transferability of this
                      optochemical toolbox to the biotechnologically relevant
                      bacteria Pseudomonas putida and Bacillus subtilis. Notably,
                      the more water‐soluble cIPTG derivative proved to be
                      particularly suitable for light‐mediated gene expression
                      in these alternative expression hosts.},
      cin          = {IBOC / IBG-1 / IMET},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBOC-20090406 / I:(DE-Juel1)IBG-1-20101118 /
                      I:(DE-Juel1)IMET-20090612},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32914927},
      UT           = {WOS:000581110400001},
      doi          = {10.1002/cbic.202000377},
      url          = {https://juser.fz-juelich.de/record/885458},
}