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@ARTICLE{Acebron:885466,
author = {Acebron, Kelvin and Matsubara, Shizue and Jedmowski,
Christoph and Emin, Dzhaner and Muller, Onno and Rascher,
Uwe},
title = {{D}iurnal dynamics of non‐photochemical quenching in
{A}rabidopsis npq mutants assessed by solar‐induced
fluorescence and reflectance measurements in the field},
journal = {The new phytologist},
volume = {229},
number = {4},
issn = {1469-8137},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2020-03849},
pages = {2104-2119},
year = {2021},
abstract = {Solar‐induced fluorescence (SIF) is highly relevant in
mapping photosynthesis from remote‐sensing platforms. This
requires linking SIF to photosynthesis and understanding the
role of nonphotochemical quenching (NPQ) mechanisms under
field conditions. Hence, active and passive fluorescence
were measured in Arabidopsis with altered NPQ in outdoor
conditions. Plants with mutations in either violaxanthin
de‐epoxidase (npq1) or PsbS protein (npq4) exhibited
reduced NPQ capacity. Parallel measurements of NPQ,
photosystem II efficiency, SIF and spectral reflectance (ρ)
were conducted diurnally on one sunny summer day and two
consecutive days during a simulated cold spell. Results
showed that both npq mutants exhibited higher levels of SIF
compared to wild‐type plants. Changes in reflectance were
related to changes in the
violaxanthin–antheraxanthin–zeaxanthin cycle and not to
PsbS‐mediated conformational changes. When plants were
exposed to cold temperatures, rapid onset of photoinhibition
strongly quenched SIF in all lines. Using
well‐characterized Arabidopsis npq mutants, we showed for
the first time the quantitative link between SIF,
photosynthetic efficiency, NPQ components and leaf
reflectance. We discuss the functional potential and
limitations of SIF and reflectance measurements for
estimating photosynthetic efficiency and NPQ in the field.},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582) / 2171 - Biological and
environmental resources for sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF3-582 / G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)16},
pubmed = {33020945},
UT = {WOS:000584802700001},
doi = {10.1111/nph.16984},
url = {https://juser.fz-juelich.de/record/885466},
}