TY  - JOUR
AU  - Graf, Michaela
AU  - Haas, Thorsten
AU  - Teleki, Attila
AU  - Feith, André
AU  - Cerff, Martin
AU  - Wiechert, Wolfgang
AU  - Nöh, Katharina
AU  - Busche, Tobias
AU  - Kalinowski, Jörn
AU  - Takors, Ralf
TI  - Revisiting the growth modulon of Corynebacterium glutamicum under glucose limited chemostat conditions
JO  - Frontiers in Bioengineering and Biotechnology
VL  - 8
SN  - 2296-4185
CY  - Lausanne
PB  - Frontiers Media
M1  - FZJ-2020-04041
SP  - 584614
PY  - 2020
AB  - Increasing the growth rate of the industrial host Corynebacterium glutamicum is a promising target to rise productivities of growth coupled product formation. As a prerequisite, detailed knowledge about the tight regulation network is necessary for identifying promising metabolic engineering goals. Here, we present comprehensive metabolic and transcriptional analysis of C. glutamicum ATCC 13032 growing under glucose limited chemostat conditions with μ = 0.2, 0.3, and 0.4 h–1. Intermediates of central metabolism mostly showed rising pool sizes with increasing growth. 13C-metabolic flux analysis (13C-MFA) underlined the fundamental role of central metabolism for the supply of precursors, redox, and energy equivalents. Global, growth-associated, concerted transcriptional patterns were not detected giving rise to the conclusion that glycolysis, pentose-phosphate pathway, and citric acid cycle are predominately metabolically controlled under glucose-limiting chemostat conditions. However, evidence is found that transcriptional regulation takes control over glycolysis once glucose-rich growth conditions are installed.
LB  - PUB:(DE-HGF)16
C6  - pmid:33178676
UR  - <Go to ISI:>//WOS:000584724900001
DO  - DOI:10.3389/fbioe.2020.584614
UR  - https://juser.fz-juelich.de/record/885729
ER  -