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@ARTICLE{Sonntag:885829,
      author       = {Sonntag, Christiane Katharina and Flachbart, Lion
                      Konstantin and Maass, Celine and Vogt, Michael and
                      Marienhagen, Jan},
      title        = {{A} unified design allows fine-tuning of biosensor
                      parameters and application across bacterial species},
      journal      = {Metabolic engineering communications},
      volume       = {11},
      issn         = {2214-0301},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2020-04120},
      pages        = {e00150 -},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {In recent years, transcriptional biosensors have become
                      valuable tools in metabolic engineering as they allow
                      semiquantitative determination of metabolites in single
                      cells. Although being perfectly suitable tools for
                      high-throughput screenings, application of transcriptional
                      biosensors is often limited by the intrinsic characteristics
                      of the individual sensor components and their interplay. In
                      addition, biosensors often fail to work properly in
                      heterologous host systems due to signal saturation at low
                      intracellular metabolite concentrations, which typically
                      limits their use in high-level producer strains at advanced
                      engineering stages.We here introduce a biosensor design,
                      which allows fine-tuning of important sensor parameters and
                      restores the sensor response in a heterologous expression
                      host. As a key feature of our design, the regulator activity
                      is controlled through the expression level of the respective
                      gene by different (synthetic) constitutive promoters
                      selected for the used expression host. In this context, we
                      constructed biosensors responding to basic amino acids or
                      ring-hydroxylated phenylpropanoids for applications in
                      Corynebacterium glutamicum and Escherichia coli. Detailed
                      characterization of these biosensors in liquid cultures and
                      during single-cell analysis using flow cytometry showed that
                      the presented sensor design enables customization of
                      important biosensor parameters as well as application of
                      these sensors in relevant heterologous hosts.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {583 - Innovative Synergisms (POF3-583) / 581 -
                      Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-583 / G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33145168},
      UT           = {WOS:000905576000021},
      doi          = {10.1016/j.mec.2020.e00150},
      url          = {https://juser.fz-juelich.de/record/885829},
}