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@ARTICLE{Fan:885877,
      author       = {Fan, Xiaoming and Gensch, Thomas and Büldt, Georg and
                      Zhang, Yuanheng and Musha, Zulipali and Zhang, Wenyuan and
                      Roncarati, Renza and Huang, Ruimin},
      title        = {{T}hree dimensional drift control at nano-scale in single
                      molecule localization microscopy},
      journal      = {Optics express},
      volume       = {28},
      number       = {22},
      issn         = {1094-4087},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {FZJ-2020-04153},
      pages        = {32750 -},
      year         = {2020},
      abstract     = {Super-resolution imaging based on single molecule
                      localization of cellular structures on nanometer scale
                      requires to record a series of wide-field or TIRF images
                      resulting in a considerable recording time (typically of
                      minutes). Therefore, sample drift becomes a critical problem
                      and will lower the imaging precision. Herein we utilized
                      morphological features of the specimen (mammalian cells)
                      itself as reference markers replacing the traditionally used
                      markers (e.g., artificial fiduciary markers, fluorescent
                      beads, or metal nanoparticles) for sample drift
                      compensation. We achieved sub-nanometer localization
                      precision <1.0 nm in lateral direction and <6.0 nm in axial
                      direction, which is well comparable with the precision
                      achieved with the established methods using artificial
                      position markers added to the specimen. Our method does not
                      require complex hardware setup, extra labelling or markers,
                      and has the additional advantage of the absence of
                      photobleaching, which caused precision decrease during the
                      course of super-resolution measurement. The achieved
                      improvement of quality and resolution in reconstructed
                      super-resolution images by application of our
                      drift-correction method is demonstrated by single molecule
                      localization-based super-resolution imaging of F-actin in
                      fixed A549 cells.},
      cin          = {IBI-1},
      ddc          = {530},
      cid          = {I:(DE-Juel1)IBI-1-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33114953},
      UT           = {WOS:000582499400039},
      doi          = {10.1364/OE.404123},
      url          = {https://juser.fz-juelich.de/record/885877},
}