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@ARTICLE{Kuepper:885909,
      author       = {Kuepper, Jannis and Otto, Maike and Dickler, Jasmin and
                      Behnken, Swantje and Magnus, Jorgen and Jäger, Gernot and
                      Blank, Lars M. and Wierckx, Nick},
      title        = {{A}daptive laboratory evolution of {P}seudomonas putida and
                      {C}orynebacterium glutamicum to enhance anthranilate
                      tolerance},
      journal      = {Microbiology},
      volume       = {166},
      number       = {11},
      issn         = {1465-2080},
      address      = {Reading},
      publisher    = {Soc.},
      reportid     = {FZJ-2020-04173},
      pages        = {},
      year         = {2020},
      note         = {Biotechnolgie 1},
      abstract     = {Microbial bioproduction of the aromatic acid anthranilate
                      (ortho-aminobenzoate) has the potential to replace its
                      current, environmentally demanding production process. The
                      host organism employed for such a process needs to fulfil
                      certain demands to achieve industrially relevant product
                      levels. As anthranilate is toxic for microorganisms, the use
                      of particularly robust production hosts can overcome issues
                      from product inhibition. The microorganisms Corynebacterium
                      glutamicum and Pseudomonas putida are known for high
                      tolerance towards a variety of chemicals and could serve as
                      promising platform strains. In this study, the resistance of
                      both wild-type strains towards anthranilate was assessed. To
                      further enhance their native tolerance, adaptive laboratory
                      evolution (ALE) was applied. Sequential batch fermentation
                      processes were developed, adapted to the cultivation demands
                      for C. glutamicum and P. putida, to enable long-term
                      cultivation in the presence of anthranilate. Isolation and
                      analysis of single mutants revealed phenotypes with improved
                      growth behaviour in the presence of anthranilate for both
                      strains. The characterization and improvement of both
                      potential hosts provide an important basis for further
                      process optimization and will aid the establishment of an
                      industrially competitive method for microbial synthesis of
                      anthranilate.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33095135},
      UT           = {WOS:000603436200004},
      doi          = {10.1099/mic.0.000982},
      url          = {https://juser.fz-juelich.de/record/885909},
}