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@ARTICLE{Zhu:885911,
      author       = {Zhu, Lingfeng and Mack, Christina and Wirtz, Astrid and
                      Kranz, Angela and Polen, Tino and Baumgart, Meike and Bott,
                      Michael},
      title        = {{R}egulation of γ-{A}minobutyrate ({GABA}) {U}tilization
                      in {C}orynebacterium glutamicum by the {P}uc{R}-{T}ype
                      {T}ranscriptional {R}egulator {G}ab{R} and by {A}lternative
                      {N}itrogen and {C}arbon {S}ources},
      journal      = {Frontiers in microbiology},
      volume       = {11},
      issn         = {1664-302X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2020-04175},
      pages        = {544045},
      year         = {2020},
      note         = {Biotechnologie 1},
      abstract     = {γ-Aminobutyric acid (GABA) is a non-proteinogenic amino
                      acid mainly formed by decarboxylation of L-glutamate and is
                      widespread in nature from microorganisms to plants and
                      animals. In this study, we analyzed the regulation of GABA
                      utilization by the Gram-positive soil bacterium
                      Corynebacterium glutamicum, which serves as model organism
                      of the phylum Actinobacteria. We show that GABA usage is
                      subject to both specific and global regulatory mechanisms.
                      Transcriptomics revealed that the gabTDP genes encoding GABA
                      transaminase, succinate semialdehyde dehydrogenase, and GABA
                      permease, respectively, were highly induced in GABA-grown
                      cells compared to glucose-grown cells. Expression of the
                      gabTDP genes was dependent on GABA and the PucR-type
                      transcriptional regulator GabR, which is encoded divergently
                      to gabT. A ΔgabR mutant failed to grow with GABA, but not
                      with glucose. Growth of the mutant on GABA was restored by
                      plasmid-based expression of gabR or of gabTDP, indicating
                      that no further genes are specifically required for GABA
                      utilization. Purified GabR (calculated mass 55.75 kDa)
                      formed an octamer with an apparent mass of 420 kDa and bound
                      to two inverted repeats in the gabR-gabT intergenic region.
                      Glucose, gluconate, and myo-inositol caused reduced
                      expression of gabTDP, presumably via the cAMP-dependent
                      global regulator GlxR, for which a binding site is present
                      downstream of the gabT transcriptional start site. C.
                      glutamicum was able to grow with GABA as sole carbon and
                      nitrogen source. Ammonium and, to a lesser extent, urea
                      inhibited growth on GABA, whereas L-glutamine stimulated it.
                      Possible mechanisms for these effects are discussed.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33193127},
      UT           = {WOS:000587701900001},
      doi          = {10.3389/fmicb.2020.544045},
      url          = {https://juser.fz-juelich.de/record/885911},
}