TY  - JOUR
AU  - Beck, Jürgen
AU  - Heymann, William
AU  - von Lieres, Eric
AU  - Hahn, Rainer
TI  - Compartment Model of Mixing in a Bubble Trap and Its Impact on Chromatographic Separations
JO  - Processes
VL  - 8
IS  - 7
SN  - 2227-9717
CY  - Basel
PB  - MDPI
M1  - FZJ-2020-04253
SP  - 780 -
PY  - 2020
AB  - Chromatography equipment includes hold-up volumes that are external to the packed bed and usually not considered in the development of chromatography models. These volumes can substantially contribute to band-broadening in the system and deteriorate the predicted performance. We selected a bubble trap of a pilot scale chromatography system as an example for a hold-up volume with a non-standard mixing behavior. In a worst-case scenario, the bubble trap is not properly flushed before elution, thus causing the significant band-broadening of the elution peak. We showed that the mixing of buffers with different densities in the bubble trap device can be accurately modeled using a simple compartment model. The model was calibrated at a wide range of flow rates and salt concentrations. The simulations were performed using the open-source software CADET, and all scripts and data are published with this manuscript. The results illustrate the importance of including external holdup volumes in chromatography modeling. The band-broadening effect of tubing, pumps, valves, detectors, frits, or any other zones with non-standard mixing behavior can be considered in very similar way
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000557505800001
DO  - DOI:10.3390/pr8070780
UR  - https://juser.fz-juelich.de/record/886066
ER  -