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@PHDTHESIS{Xing:886095,
author = {Xing, Ying},
title = {{I}ron isotope fractionation in arable soil and
graminaceous crops},
volume = {517},
school = {Universität Bonn},
type = {Dissertation},
address = {Jülich},
publisher = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
reportid = {FZJ-2020-04263},
isbn = {978-3-95806-509-3},
series = {Schriften des Forschungszentrums Jülich. Reihe Energie
$\&$ Umwelt / Energy $\&$ Environment},
pages = {123 S.},
year = {2020},
note = {Universität Bonn, Diss., 2020},
abstract = {Soils contain large quantities of Fe, however, the
Fe-solubility is very low. Plants have developed two
efficient strategies to secure Fe uptake from soil under
Fe-deficient conditions: (i) the sequential
acidification-reduction-transport strategy (strategy I) and
(ii) the chelation-based strategy (strategy II). All
processes involved in the Fe cycle in soil-plant systems can
fractionate stable Fe isotopes. Hence, I (i) conducted a
systematic review about the state of Fe isotope researchin
plant studies and highlighted the research gaps. Then I
supplemented this theoretical study by two experiments: I
(ii) examined the effect of different Fe availabilities on
Fe isotope fractionation in wheat plants under controlled
conditions and I (ii) investigated the effect of 50 years of
irrigation on Fe isotope fractionation in soils and cereals
in a long-term field experiment. My review suggested that
strategy I plants especially take up light Fe isotopes,
while strategy II plants fractionate less towards light
isotopes. Above ground tissues usually show even lighter Fe
isotope signatures than the roots, with flowers
(δ$^{56}$Fe: -2.15 to -0.23‰) being isotopically the
lightest. I found that all reported strategy I plants
consistently enriched light Fe isotopes under all growth
conditions. Strategy II plants, however, could be enriched
with either light or heavy Fe isotopes, depending on the
growth conditions. Depending on the Fe speciation and
concentration present in the growth medium, some strategy II
plants like rice are able to adapt their uptake strategy as
they also possess ferrous transporters and are hence also
able to take up Fe(II) ions. In a greenhouse study, I
cultivated summer wheat ($\textit{Triticum aestivum L.}$)
under Fe-sufficient(control, 0.0896 mM Fe-EDTA) and
deficient (Fe-deficient, 0.0022 mM Fe-EDTA) conditions.
Plants were sampled at different growth stages (vegetative
and reproductive growth stages) and separated into different
plant organs (root, stem, leaf, spike/grain). All samples
were analyzed for their Fe concentrations and δ$^{56}$Fe
isotope compositions. The results showed that Fe-deficiency
reduced the whole plant Fe mass by 59\% at vegetative
growth. During reproductive growth, Femass fluxes indicated
different preferential Fe translocation pathways under
different Fe supply. Under Fe-deficient conditions, Fe
uptake from growth substrate increased whereas under Fe
sufficient conditions Fe was preferentially redistributed
within the plant. Under Fe-sufficient conditions
increasingly lighter δ$^{56}$Fe values from older to
younger plant parts were found, but no indications that the
chelation-based uptake strategy was activated. However, with
serious shortage of Fe, the shift towards lighter
δ$^{56}$Fe values was reduced. This suggested that Fe
isotope ratios can reflect both wheat growth conditions and
ages. In a field study, I sampled wheat plants and Retisol
soil cores down to a depth of 100 cm from along-term
irrigation treatment at Berlin-Thyrow. The irrigated plots
had higher Fe avail concentrations than the non-irrigated
plots in the top 40 cm of soil, but there were no changes in
δ$^{56}$Fe values. Due to the research site being one of
the driest areas in Germany with hardly ameaningful water
percolation, the maximum difference of δ$^{56}$Fe$_{avail}$
values between 40 to 50 cm and 70 to 100 cm was explained
soil pedogenesis rather than irrigation treatment. The wheat
plants grown in both irrigated and non-irrigated plots were
slightly enriched in light Fe isotopes, exhibiting similar
δ$^{56}$Fe values to those of the respective topsoil. I
concluded that the overall δ $^{56}$Fesignature of wheat
was regulated by plant-homeostasis and specific on-site soil
characteristics, whereas irrigation had little if any
significant effect on the Fe isotopes in the crops. Overall,
my study showed that the Fe isotope compositions of wheat
plants were not affected by Fe availabilities in substrate
until the anthesis stage. However, during the reproductive
growth phase with sufficient Fe supply, δ$^{56}$Fe values
of different plant organs showed significant Fe
fractionation. The former processes were hardly affected by
irrigation.},
cin = {IBG-3},
cid = {I:(DE-Juel1)IBG-3-20101118},
pnm = {255 - Terrestrial Systems: From Observation to Prediction
(POF3-255)},
pid = {G:(DE-HGF)POF3-255},
typ = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
urn = {urn:nbn:de:0001-2020120304},
url = {https://juser.fz-juelich.de/record/886095},
}