%0 Journal Article
%A Wehrmann, Matthias
%A Elsayed, Eslam M.
%A Köbbing, Sebastian
%A Bendz, Laura
%A Lepak, Alexander
%A Schwabe, Johannes
%A Wierckx, Nick
%A Bange, Gert
%A Klebensberger, Janosch
%T Engineered PQQ-Dependent Alcohol Dehydrogenase for the Oxidation of 5-(Hydroxymethyl)furoic Acid
%J ACS catalysis
%V 10
%N 14
%@ 2155-5435
%C Washington, DC
%I ACS
%M FZJ-2020-04296
%P 7836 - 7842
%D 2020
%Z Biotechnologie 1   
%X Furan-2,5-dicarboxylic acid (FDCA) is a bio-based platform chemical with the potential to replace terephthalic acid in the production of polymers. A critical step for enzymatic and whole-cell production of FDCA from 5-(hydroxymethyl)furfural (HMF) is the transformation of 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA). Here, we establish periplasmic pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenases (ADHs) as biocatalytic tools for the oxidation of HMFA, HMF, and 5-formylfurfural (FFF). Further, we identify several amino acid residues including the “lid loop” of the substrate channel as promising targets for future engineering steps toward a fully periplasmic oxidation pathway to FDCA.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000551549800035
%R 10.1021/acscatal.0c01789
%U https://juser.fz-juelich.de/record/886135