TY  - JOUR
AU  - Wehrmann, Matthias
AU  - Elsayed, Eslam M.
AU  - Köbbing, Sebastian
AU  - Bendz, Laura
AU  - Lepak, Alexander
AU  - Schwabe, Johannes
AU  - Wierckx, Nick
AU  - Bange, Gert
AU  - Klebensberger, Janosch
TI  - Engineered PQQ-Dependent Alcohol Dehydrogenase for the Oxidation of 5-(Hydroxymethyl)furoic Acid
JO  - ACS catalysis
VL  - 10
IS  - 14
SN  - 2155-5435
CY  - Washington, DC
PB  - ACS
M1  - FZJ-2020-04296
SP  - 7836 - 7842
PY  - 2020
N1  - Biotechnologie 1   
AB  - Furan-2,5-dicarboxylic acid (FDCA) is a bio-based platform chemical with the potential to replace terephthalic acid in the production of polymers. A critical step for enzymatic and whole-cell production of FDCA from 5-(hydroxymethyl)furfural (HMF) is the transformation of 5-(hydroxymethyl)furoic acid (HMFA) into 5-formylfuroic acid (FFA). Here, we establish periplasmic pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenases (ADHs) as biocatalytic tools for the oxidation of HMFA, HMF, and 5-formylfurfural (FFF). Further, we identify several amino acid residues including the “lid loop” of the substrate channel as promising targets for future engineering steps toward a fully periplasmic oxidation pathway to FDCA.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000551549800035
DO  - DOI:10.1021/acscatal.0c01789
UR  - https://juser.fz-juelich.de/record/886135
ER  -