guest ::
| Home > Publications database > Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein > print |
| Home > Publications database > Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein > print |
| 001 | 887775 | ||
| 005 | 20210130010635.0 | ||
| 024 | 7 | _ | |a 10.1021/acs.jpclett.9b03252 |2 doi |
| 024 | 7 | _ | |a 2128/26451 |2 Handle |
| 024 | 7 | _ | |a altmetric:73705210 |2 altmetric |
| 024 | 7 | _ | |a pmid:31880458 |2 pmid |
| 024 | 7 | _ | |a WOS:000508473400022 |2 WOS |
| 037 | _ | _ | |a FZJ-2020-04412 |
| 082 | _ | _ | |a 530 |
| 100 | 1 | _ | |a Shibazaki, Chie |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Direct Observation of the Protonation States in the Mutant Green Fluorescent Protein |
| 260 | _ | _ | |a Washington, DC |c 2020 |b ACS |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1607523754_30926 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Neutron crystallography has been used to elucidate the protonationstates for the enhanced green fluorescent protein, which has revolutionized imagingtechnologies. The structure has a deprotonated hydroxyl group in the fluorescentchromophore. Also, the protonation states of His148 and Thr203, as well as theorientation of a critical water molecule in direct contact with the chromophore, couldbe determined. The results demonstrate that the deprotonated hydroxyl group in thechromophore and the nitrogen atom ND1 in His148 are charged negatively andpositively, respectively, forming an ion pair. The position of the two deuterium atomsin the critical water molecule appears to be displaced slightly toward the acceptoroxygen atoms according to their omit maps. This displacement implies the formationof an intriguing electrostatic potential realized inside of the protein. Our findingsprovide new insights into future protein design strategies along with developments inquantum chemical calculations. |
| 536 | _ | _ | |a 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) |0 G:(DE-HGF)POF3-6G4 |c POF3-623 |f POF III |x 0 |
| 536 | _ | _ | |0 G:(DE-HGF)POF3-6G15 |f POF III |x 1 |c POF3-6G15 |a 6G15 - FRM II / MLZ (POF3-6G15) |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 650 | 2 | 7 | |a Biology |0 V:(DE-MLZ)SciArea-160 |2 V:(DE-HGF) |x 0 |
| 650 | 1 | 7 | |a Health and Life |0 V:(DE-MLZ)GC-130-2016 |2 V:(DE-HGF) |x 0 |
| 693 | _ | _ | |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz |e BIODIFF: Diffractometer for large unit cells |f NL1 |1 EXP:(DE-MLZ)FRMII-20140101 |0 EXP:(DE-MLZ)BIODIFF-20140101 |5 EXP:(DE-MLZ)BIODIFF-20140101 |6 EXP:(DE-MLZ)NL1-20140101 |x 0 |
| 700 | 1 | _ | |a Shimizu, Rumi |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Kagotani, Yuji |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Ostermann, Andreas |0 0000-0002-1477-5590 |b 3 |
| 700 | 1 | _ | |a Schrader, Tobias E. |0 P:(DE-Juel1)138266 |b 4 |
| 700 | 1 | _ | |a Adachi, Motoyasu |0 0000-0003-2353-880X |b 5 |e Corresponding author |
| 773 | _ | _ | |a 10.1021/acs.jpclett.9b03252 |0 PERI:(DE-600)2522838-9 |p 492-496 |t The journal of physical chemistry letters |v 11 |y 2020 |x 1948-7185 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/887775/files/acs.jpclett.9b03252.pdf |
| 856 | 4 | _ | |y Published on 2019-12-27. Available in OpenAccess from 2020-12-27. |u https://juser.fz-juelich.de/record/887775/files/schrader_acs.jpclett.9b03252.pdf |
| 909 | C | O | |o oai:juser.fz-juelich.de:887775 |p openaire |p open_access |p driver |p VDB:MLZ |p VDB |p dnbdelivery |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)138266 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Materie |l Von Materie zu Materialien und Leben |1 G:(DE-HGF)POF3-620 |0 G:(DE-HGF)POF3-623 |2 G:(DE-HGF)POF3-600 |v Facility topic: Neutrons for Research on Condensed Matter |9 G:(DE-HGF)POF3-6G4 |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 913 | 1 | _ | |a DE-HGF |9 G:(DE-HGF)POF3-6G15 |x 1 |4 G:(DE-HGF)POF |v FRM II / MLZ |1 G:(DE-HGF)POF3-6G0 |0 G:(DE-HGF)POF3-6G15 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-600 |b Forschungsbereich Materie |l Großgeräte: Materie |
| 914 | 1 | _ | |y 2020 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2020-09-03 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2020-09-03 |
| 915 | _ | _ | |a Embargoed OpenAccess |0 StatID:(DE-HGF)0530 |2 StatID |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b J PHYS CHEM LETT : 2018 |d 2020-09-03 |
| 915 | _ | _ | |a IF >= 5 |0 StatID:(DE-HGF)9905 |2 StatID |b J PHYS CHEM LETT : 2018 |d 2020-09-03 |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2020-09-03 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2020-09-03 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1150 |2 StatID |b Current Contents - Physical, Chemical and Earth Sciences |d 2020-09-03 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2020-09-03 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2020-09-03 |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)JCNS-FRM-II-20110218 |k JCNS-FRM-II |l JCNS-FRM-II |x 0 |
| 920 | 1 | _ | |0 I:(DE-Juel1)JCNS-1-20110106 |k JCNS-1 |l Neutronenstreuung |x 1 |
| 920 | 1 | _ | |0 I:(DE-588b)4597118-3 |k MLZ |l Heinz Maier-Leibnitz Zentrum |x 2 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)JCNS-FRM-II-20110218 |
| 980 | _ | _ | |a I:(DE-Juel1)JCNS-1-20110106 |
| 980 | _ | _ | |a I:(DE-588b)4597118-3 |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|