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@ARTICLE{RincnMontes:887905,
author = {Rincón Montes, V. and Gehlen, Jana and Ingebrandt, S. and
Mokwa, W. and Walter, P. and Müller, Frank and
Offenhäusser, A.},
title = {{D}evelopment and in vitro validation of flexible
intraretinal probes},
journal = {Scientific reports},
volume = {10},
issn = {2045-2322},
address = {[London]},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2020-04511},
pages = {19836},
year = {2020},
abstract = {The efforts to improve the treatment efficacy in blind
patients with retinal degenerative diseases would greatly
benefit from retinal activity feedback, which is lacking in
current retinal implants. While the door for a bidirectional
communication device that stimulates and records
intraretinally has been opened by the recent use of
silicon-based penetrating probes, the biological impact
induced by the insertion of such rigid devices is still
unknown. Here, we developed for the first time, flexible
intraretinal probes and validated in vitro the acute
biological insertion impact in mouse retinae compared to
standard silicon-based probes. Our results show that probes
based on flexible materials, such as polyimide and
parylene-C, in combination with a narrow shank design 50 µm
wide and 7 µm thick, and the use of insertion speeds as
high as 187.5 µm/s will successfully penetrate the retina,
reduce the footprint of the insertion to roughly 2 times the
cross-section of the probe, and induce low dead cell counts,
while keeping the vitality of the tissue and recording the
neural activity at different depths.},
cin = {IBI-1},
ddc = {600},
cid = {I:(DE-Juel1)IBI-1-20200312},
pnm = {553 - Physical Basis of Diseases (POF3-553)},
pid = {G:(DE-HGF)POF3-553},
typ = {PUB:(DE-HGF)16},
pubmed = {33199768},
UT = {WOS:000595259200008},
doi = {10.1038/s41598-020-76582-5},
url = {https://juser.fz-juelich.de/record/887905},
}