TY  - JOUR
AU  - Lenyk, Bohdan
AU  - Figueroa‐Miranda, Gabriela
AU  - Pavlushko, Ivan
AU  - Lo, Young
AU  - Tanner, Julian A.
AU  - Offenhäusser, Andreas
AU  - Mayer, Dirk
TI  - Dual‐Transducer Malaria Aptasensor Combining Electrochemical Impedance and Surface Plasmon Polariton Detection on Gold Nanohole Arrays
JO  - ChemElectroChem
VL  - 7
IS  - 22
SN  - 2196-0216
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2020-04532
SP  - 4594 - 4600
PY  - 2020
AB  - Two transducer principles are combined in one aptamer biosensor (aptasensor) by simultaneously performing electrochemical impedance spectroscopy (EIS) and surface plasmon polariton (SPP) detection of a malaria biomarker. A thin gold film perforated with nanohole arrays is modified with small and highly charged aptamer receptors and utilized for the detection of Plasmodium falciparum lactate dehydrogenase (PfLDH), the main biomarker of malaria. Monitoring the same analyte binding events by two independent transduction principles not only corroborates the in situ detection, but also covers a concentration range of six orders of magnitude (1 pM–1 μM). The EIS method is highly sensitive to low concentrations of PfLDH (1 pM–100 nM), whereas SPP is sensitive to higher concentrations of the target (10 nM–1 μM), owing to either high interfacial or more bulk sensitivity, respectively. Thus, we propose the dual‐transducer aptasensor based on gold nanohole arrays as a platform for a broad dynamic concentration range and reliable detection.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000593989300010
DO  - DOI:10.1002/celc.202001212
UR  - https://juser.fz-juelich.de/record/887940
ER  -