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@ARTICLE{Meixner:888236,
author = {Meixner, Marco and Tomasella, Martina and Foerst, Petra and
Windt, Carel W.},
title = {{A} small‐scale {MRI} scanner and complementary imaging
method to visualize and quantify xylem embolism formation},
journal = {The new phytologist},
volume = {226},
number = {5},
issn = {1469-8137},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2020-04785},
pages = {1517 - 1529},
year = {2020},
abstract = {Magnetic resonance imaging (MRI) is a useful tool to image
xylem embolism formation in plants. MRI scanners configured
to accept intact plants are rare and expensive. Here, we
investigate if affordable small-scale, custom-built
low-field MRI scanners would suffice for the purpose. * A
small-scale, C-shaped permanent magnet was paired with open,
plane parallel imaging gradients. The setup was small enough
to fit between leaves or branches and offered open access
for plant stems of arbitrary length. To counter the two main
drawbacks of the system, low signal to noise and reduced
magnetic field homogeneity, a multi-spin echo (MSE) pulse
sequence was implemented, allowing efficient signal
acquisition and quantitative imaging of water content and T2
signal relaxation. * The system was tested visualizing
embolism formation in Fagus sylvatica during bench
dehydration. High-quality images of water content and T2
were readily obtained, which could be utilized to detect the
cavitation of vessels smaller than could be spatially
resolved. A multiplication of both map types yielded images
in which filled xylem appeared with even greater contrast. *
T2 imaging with small-scale MRI devices allows
straightforward visualization of the spatial and temporal
dynamics of embolism formation and the derivation of
vulnerability curves.},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)16},
pubmed = {31958150},
UT = {WOS:000517614900001},
doi = {10.1111/nph.16442},
url = {https://juser.fz-juelich.de/record/888236},
}