TY  - JOUR
AU  - Zabelskii, Dmitrii
AU  - Alekseev, Alexey
AU  - Kovalev, Kirill
AU  - Rankovic, Vladan
AU  - Balandin, Taras
AU  - Soloviov, Dmytro
AU  - Bratanov, Dmitry
AU  - Savelyeva, Ekaterina
AU  - Podolyak, Elizaveta
AU  - Volkov, Dmytro
AU  - Vaganova, Svetlana
AU  - Astashkin, Roman
AU  - Chizhov, Igor
AU  - Yutin, Natalia
AU  - Rulev, Maksim
AU  - Popov, Alexander
AU  - Eria-Oliveira, Ana-Sofia
AU  - Rokitskaya, Tatiana
AU  - Mager, Thomas
AU  - Antonenko, Yuri
AU  - Rosselli, Riccardo
AU  - Armeev, Grigoriy
AU  - Shaitan, Konstantin
AU  - Vivaudou, Michel
AU  - Büldt, Georg
AU  - Rogachev, Andrey
AU  - Rodriguez-Valera, Francisco
AU  - Kirpichnikov, Mikhail
AU  - Moser, Tobias
AU  - Offenhäusser, Andreas
AU  - Willbold, Dieter
AU  - Koonin, Eugene
AU  - Bamberg, Ernst
AU  - Gordeliy, Valentin
TI  - Viral rhodopsins 1 are an unique family of light-gated cation channels
JO  - Nature Communications
VL  - 11
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - FZJ-2020-04786
SP  - 5707
PY  - 2020
AB  - Phytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct families. Here, we present a functional and structural characterization of two proteins of viral rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis of VirChR1 shows that it is a highly selective, Na+/K+-conducting channel and, in contrast to known cation channelrhodopsins, it is impermeable to Ca2+ ions. We show that, upon illumination, VirChR1 is able to drive neural firing. The 1.4 Å resolution structure of OLPVR1 reveals remarkable differences from the known channelrhodopsins and a unique ion-conducting pathway. Thus, viral rhodopsins 1 represent a unique, large group of light-gated channels (viral channelrhodopsins, VirChR1s). In nature, VirChR1s likely mediate phototaxis of algae enhancing the host anabolic processes to support virus reproduction, and therefore, might play a major role in global phytoplankton dynamics. Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca2+ permeability.
LB  - PUB:(DE-HGF)16
C6  - pmid:33177509
UR  - <Go to ISI:>//WOS:000593975100009
DO  - DOI:10.1038/s41467-020-19457-7
UR  - https://juser.fz-juelich.de/record/888237
ER  -