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@ARTICLE{Zabelskii:888237,
author = {Zabelskii, Dmitrii and Alekseev, Alexey and Kovalev, Kirill
and Rankovic, Vladan and Balandin, Taras and Soloviov,
Dmytro and Bratanov, Dmitry and Savelyeva, Ekaterina and
Podolyak, Elizaveta and Volkov, Dmytro and Vaganova,
Svetlana and Astashkin, Roman and Chizhov, Igor and Yutin,
Natalia and Rulev, Maksim and Popov, Alexander and
Eria-Oliveira, Ana-Sofia and Rokitskaya, Tatiana and Mager,
Thomas and Antonenko, Yuri and Rosselli, Riccardo and
Armeev, Grigoriy and Shaitan, Konstantin and Vivaudou,
Michel and Büldt, Georg and Rogachev, Andrey and
Rodriguez-Valera, Francisco and Kirpichnikov, Mikhail and
Moser, Tobias and Offenhäusser, Andreas and Willbold,
Dieter and Koonin, Eugene and Bamberg, Ernst and Gordeliy,
Valentin},
title = {{V}iral rhodopsins 1 are an unique family of light-gated
cation channels},
journal = {Nature Communications},
volume = {11},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {FZJ-2020-04786},
pages = {5707},
year = {2020},
abstract = {Phytoplankton is the base of the marine food chain as well
as oxygen and carbon cycles and thus plays a global role in
climate and ecology. Nucleocytoplasmic Large DNA Viruses
that infect phytoplankton organisms and regulate the
phytoplankton dynamics encompass genes of rhodopsins of two
distinct families. Here, we present a functional and
structural characterization of two proteins of viral
rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis
of VirChR1 shows that it is a highly selective,
Na+/K+-conducting channel and, in contrast to known cation
channelrhodopsins, it is impermeable to Ca2+ ions. We show
that, upon illumination, VirChR1 is able to drive neural
firing. The 1.4 Å resolution structure of OLPVR1 reveals
remarkable differences from the known channelrhodopsins and
a unique ion-conducting pathway. Thus, viral rhodopsins 1
represent a unique, large group of light-gated channels
(viral channelrhodopsins, VirChR1s). In nature, VirChR1s
likely mediate phototaxis of algae enhancing the host
anabolic processes to support virus reproduction, and
therefore, might play a major role in global phytoplankton
dynamics. Moreover, VirChR1s have unique potential for
optogenetics as they lack possibly noxious Ca2+
permeability.},
cin = {IBI-7 / IBI-3},
ddc = {500},
cid = {I:(DE-Juel1)IBI-7-20200312 / I:(DE-Juel1)IBI-3-20200312},
pnm = {581 - Biotechnology (POF3-581) / 552 - Engineering Cell
Function (POF3-552)},
pid = {G:(DE-HGF)POF3-581 / G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33177509},
UT = {WOS:000593975100009},
doi = {10.1038/s41467-020-19457-7},
url = {https://juser.fz-juelich.de/record/888237},
}